国家重点基础研究发展计划(973计划);国家自然科学基金项目(面上项目,重点项目,重大项目)
The National Basic Research Program of China (973 Program);The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
以28个野生老芒麦材料为研究对象,于2013-2014年对落粒率等15个农艺性状进行田间观测,采用SPSS 19.0数据分析软件对观测指标进行聚类、主成分和相关性分析,并利用隶属函数法对其农艺性状进行综合评价。结果表明:老芒麦在盛花期后31d,各材料的落粒率显著增加,达到最大。其中ZhN03和MQ01的变化表现最为突出,ZhN02、HZ03和LQ05的落粒率在各单株间波动明显。两年落粒观测数据表明XH09的落粒率最高,其次为XH03、LQ03和LQ04。落粒率最低的材料为ZhN03,MQ01次之。相关分析表明落粒率与旗叶宽(-0.47)和茎节数(-0.44)呈显著负相关,与倒二叶宽(-0.50)呈极显著负相关。其余各观测性状也存在较大变异,分蘖数在所有评价指标中波动性表现最大,变异系数为17.07%。隶属函数分析表明,ZhN03的综合性状隶属函数值为0.73,最接近于1,在28种材料中农艺综合性状最为优异。本研究筛选的低落粒老芒麦种质和综合性状优异材料为老芒麦育种提供了宝贵的遗传和基因资源。
In this study, 28 E. sibiricus accessions were selected and evaluated for agronomic performance in 15 traits in order to screen the materials with low seed shattering rate and excellent agronomic traits. The cluster analysis, correlative analysis, and component analysis were conducted by SPSS 19.0. The subordinate function was used for the comprehensive evaluation of agronomic traits. The results indicated the higher seed shattering rate were found at about 31 days after flowering. Variation of seed shattering varied in tested accessions, great variation were found in ZhN03 and MQ01. Meanwhile, seed shattering of individual plant within each accessions varied as well. Based two years’ data, XH09 had the highest seed shattering rate, followed by XH03, LQ03 and LQ04; ZhN03 and MQ01 had relatively low seed shattering rate. The correlative analysis revealed that significant negative correlation exist between shattered rate and flag leaf width(-0.47), shattered rate and internodes number(-0.44); Length of the second leaf from the inflorescence is highly significant negative correlative with the shattered rate (-0.50). Phenotypic variation were also found in other traits, of which tiller number has the greatest variation with the CV of 17.7%. The subordinative function showed ZhN03 had the best comprehensive performance with subordinate function value of 0.73. These screened materials with low seed shattering rate and excellent agronomic performance are important materials for future breeding programs.
赵旭红,姜旭,赵凯,等.低落粒老芒麦种质筛选及农艺性状综合评价[J].植物遗传资源学报,2015,16(4):691-699.
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