引用本文
  • 孙梦涵,邢宝,崔宏亮,等.藜麦种质资源遗传多样性SSR标记分析[J].植物遗传资源学报,2021,22(3):625-637.    [点击复制]
  • SUN Meng-han,XING Bao,CUI Hong-liang,et al.Genetic Diversity Analysis of Quinoa by SSR Markers[J].植物遗传资源学报,2021,22(3):625-637.   [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 320次   下载 230 本文二维码信息
码上扫一扫!
藜麦种质资源遗传多样性SSR标记分析
孙梦涵1, 邢宝2, 崔宏亮3, 周帮伟4, 张琴萍1, 任贵兴1, 秦培友2
0
(1.成都大学农业农村部杂粮加工重点实验室;2.中国农业科学院作物科学研究所;3.伊犁哈萨克自治州农业科学研究所;4.东北师范大学草地科学研究所/植被生态科学教育部重点实验室)
摘要:
为研究藜麦(Chenopodium quinoa Willd.)种质资源的遗传多样性,分析国内藜麦种质遗传背景,本研究利用66个简单重复序列(SSR)标记对163份藜麦种质和3份台湾红藜(Chenopodium formosanum Koidz.)种质进行分子标记,分析了该种质群体的多态性和亲缘关系。数据显示,66对SSR标记在166份种质材料中检测到327个等位位点,平均每个标记5.031个等位位点,平均观测杂合度和期望杂合度分别为0.387和0.588,平均多态性信息含量为0.524。用类平均法将166份材料聚为3个组群,其中Ⅰ组仅包括3份台湾红藜,Ⅱ组包括以来源于美国国家种质库和智利种质为主的103份种质材料,Ⅲ组包括以来源于玻利维亚和秘鲁种质为主的60份种质材料。群体结构分析和主成分分析将藜麦群体划分为两个亚群,亚群之间有基因交流。结果表明,玻利维亚和秘鲁种质与美国和智利种质的遗传信息存在明显区分,来自青海和云南的藜麦种质在亲缘关系上更接近安第斯高原型,来自河北、山西的藜麦种质更接近智利低海拔型。台湾红藜为台湾本土种质。
关键词:  藜麦  SSR标记  遗传多样性  聚类分析  群体结构
DOI:10.13430/j.cnki.jpgr.20200911001
投稿时间:2020-09-11修订日期:2020-10-10
基金项目:中国农业科学院科技创新工程专项经费(CAAS-ASTIP-2017-ICS)
Genetic Diversity Analysis of Quinoa by SSR Markers
SUN Meng-han1, XING Bao2, CUI Hong-liang3, ZHOU Bang-wei4, ZHANG Qin-ping1, REN Gui-xing1, QIN Pei-you2
(1.Chengdu University Key Laboratory of Minor Cereal Processing, Ministry of Agriculture and Rural Affairs;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences;3.Institute of Agricultural Sciences of Ili Kazak Autonomous Prefecture;4.Ministry of Education Key Laboratory of Vegetation Ecology / Institute of Grassland Sciences, Northeast Normal University)
Abstract:
In order to study the genetic diversity and the genetic background of quinoa (Chenopodium quinoa Willd.) germplasms distributed in China, molecular markers research was carried out based on 163 quinoa accessions and 3 djulis (Chenopodium formosanum Koidz.) accessions by using 66 simple repeat sequence (SSR) markers, to analyze the polymorphism and inter-species relationship. According to the data, a total of 327 alleles were amplified from 66 SSR markers, with an average of 5.031 alleles per marker. The mean values of observed and expected heterozygosity were 0.387 and 0.588, respectively, while the mean values of the polymorphism information index was 0.524. The method of UPGMA clustered all the accessions into three groups. Group I included 3 djulis accessions only. Group II included 103 accessions, most of which were derived from USDA-NPGS and Chilean types. Group III included 60 accessions, most of which were derived from Bolivian and Peru types. The quinoa population was divided into two groups by population structure analysis and principal component analysis, and there was gene exchange between the two groups. The results indicate that there is a clear distinction between Bolivian, Peru accessions and American, Chilean accessions in genetic information, and the genetic relationship of the accessions from Qinghai and Yunnan are closer to Andean highland type, while the accessions from Hebei and Shanxi are closer to Chilean lowland type. Djulis is a native plant in Taiwan.
Key words:  quinoa  SSR marker  genetic diversity  cluster analysis  population structure

用微信扫一扫

用微信扫一扫