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  • 王伟伟,马锦绣,曹志琛,等.小麦TaMYB35基因的克隆与表达分析[J].植物遗传资源学报,2021,22(3):771-781.    [点击复制]
  • wangweiwei,马锦绣,曹志琛,et al.Cloning and expression analysis of TaMYB35 gene in common wheat (Triticum aestivum L.)[J].植物遗传资源学报,2021,22(3):771-781.   [点击复制]
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小麦TaMYB35基因的克隆与表达分析
王伟伟, 马锦绣, 曹志琛, 郝小聪, 朱文根, 左静红, 郭春曼, 赵昌平, 汪德州, 唐益苗
0
(北京市农林科学院北京杂交小麦工程技术研究中心/杂交小麦分子遗传北京市重点实验室)
摘要:
MYB类转录因子是高等植物中最大的转录因子家族之一,参与植物多种生命活动的调控。近年来的研究表明,MYB类转录因子在花发育过程的各个阶段以及花器官的不同部分发挥调控作用。为研究MYB转录因子在小麦(Triticum aestivum L.)穗部发育中的作用,对小麦最新基因组数据进行分析,以拟南芥花药发育调控基因AtMYB35通过同源克隆法获得小麦直系同源MYB基因,根据其染色体位置分别命名为TaMYB35A、TaMYB35B、TaMYB35D,并对其进行了分析。序列分析表明:TaMYB35A、TaMYB35B和TaMYB35D分别含有924bp、927bp和927bp的完整开放阅读框,分别编码307个、308个和308个氨基酸;TaMYB35A、TaMYB35B和TaMYB35D蛋白序列N端均含有两个MYB结构域,为MYB基因家族中的R2R3-MYB类转录因子。系统进化分析表明:TaMYB35A与二粒小麦(Triticum dicoccum)处于同一分支,其亲缘关系更近;TaMYB35B和TaMYB35D处于同一分支,亲缘关系更近。荧光定量表达分析表明:TaMYB35A、TaMYB35B和TaMYB35D主要在雄蕊中表达,小穗(除去雄蕊)中少量表达,其他组织基本不表达。不育和可育环境下,TaMYB35A表达有显著差异。本研究明确了TaMYB35在小麦花药发育过程中的表达特性,为研究二系杂交小麦分子育种提供新基因资源。
关键词:  小麦  TaMYB35  细胞程序性死亡  雄性不育
DOI:10.13430/j.cnki.jpgr.20200915001
投稿时间:2020-09-15修订日期:2020-12-04
基金项目:北京市农林科学院青年科研基金(QNJJ201805);北京市农林科学院能力建设专项(KJCX20200423);基因组学育种协同创新(KJCX201907-2)
Cloning and expression analysis of TaMYB35 gene in common wheat (Triticum aestivum L.)
wangweiwei, 马锦绣, 曹志琛, 郝小聪, 朱文根, 左静红, 郭春曼
(Beijing Hybrid Wheat Engineering Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences)
Abstract:
MYB transcription factors are one of the largest transcription factor families in higher plants, and they are involved in the regulation of a variety of plant life activities. Recent studies have shown that MYB transcription factors play a regulatory role in various stages of flower development and different parts of flower organs. In order to study the role of MYB transcription factor involved in ear development in wheat (Triticum aestivum L.), we isolated the TaMYB35 gene by taking advantage of the latest wheat genome data and the sequence homolog with the Arabidopsis thaliana AtMYB35 gene, which allowed identifying three copies TaMYB35A, TaMYB35B and TaMYB35D. Sequence analysis showed that TaMYB35A, TaMYB35B and TaMYB35D contained complete open reading frames of 924bp, 927bp and 927bp, respectively, encoding for 307, 308 and 308 amino acids, respectively. All the three members contained two MYB domains at the N-terminus, which are the feature of the R2R3-MYB transcription factor in the MYB gene family. Phylogenetic analysis showed that TaMYB35A was grouped with the orthologue of Triticum dicoccum, independent of a phylogenetic cluster where TaMYB35B and TaMYB35D were resided. TaMYB35A, TaMYB35B and TaMYB35D were found with higher transcripts in stamens, less in spikelets (excluding stamens) and no detectable transcripts in other tissues. Moreover, a significant difference in the expression of TaMYB35A was observed in samples harvested at sterile and fertile environments. Thus, this study clarified transcriptional characteristics of TaMYB35 in the development of wheat anthers, which thus provided insight for deciphering the molecular basis in wheat breeding with two-line hybrid strategy.
Key words:  Wheat  TaMYB35  Programmed cell death  Male sterility

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