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  • 雷雨,段继华,黄飞毅,等.茶树杂交F1真假杂种的SSR鉴定及遗传多样性分析[J].植物遗传资源学报,2021,22(3):748-757.    [点击复制]
  • Lei Yu,DUAN Ji-hua,HUANG Fei-yi,et al.Identification and Genetic Diversity of Tea F1 Hybrids Based on SSR Markers[J].植物遗传资源学报,2021,22(3):748-757.   [点击复制]
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茶树杂交F1真假杂种的SSR鉴定及遗传多样性分析
雷雨, 段继华, 黄飞毅, 康彦凯, 罗意, 陈宇宏, 丁玎, 姚利娜, 董丽娟, 李赛君
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(湖南省农业科学院茶叶研究所/国家茶树改良中心湖南分中心/农业部湖南茶树及茶叶观测试验站/湖南省茶树品种与种苗工程技术研究中心)
摘要:
利用EST-SSR分子标记对以福鼎大白茶和安徽1号为母本,保靖黄金茶1号为父本的两个杂交F1进行真假杂种的鉴定和遗传多样性分析,结果表明:以福鼎大白茶为母本的48株F1中共鉴定出真杂种41株,杂种率为85.42%,以安徽1号为母本的44个F1中共鉴定出真杂种35株,杂种率79.55%。筛选出的17对SSR引物在两个杂交群体中共享等位基因41个,平均含有2.82(福鼎大白茶)和2.71(安徽1号)个,具有相对较高的多态性。两个杂交F1群体的遗传多样性参数差别不大,Nei、He、Ho、I平均值分别为0.56、0.78、0.43、0.93(福鼎大白茶)和0.55、0.75、0.43、0.90(安徽1号)。福鼎大白茶×保靖黄金茶1号和安徽1号×保靖黄金茶1号杂交F1中遗传相似系数的变化幅度分别在0.44~0.90和0.48~0.95之间,具有较丰富的遗传变异。根据相似系数矩阵按UPGMA进行聚类,2个杂交群体分别在相似系数为0.60和0.64处分为三大类:两个杂交子代各先聚为一类,然后再与母本聚为一类,最后与父本聚为一类,说明这两个杂交F1代具有偏母本遗传倾向。该研究表明EST-SSR可应用于茶树真假杂种的鉴定。
关键词:  茶树  SSR  杂种鉴定  遗传多样性
DOI:10.13430/j.cnki.jpgr.20200919002
投稿时间:2020-09-19修订日期:2020-10-15
基金项目:湖南省自然科学基金(2018JJ3268);中央引导地方资金(2019XF5041);湖南省农业科技创新项目(2020CX035)
Identification and Genetic Diversity of Tea F1 Hybrids Based on SSR Markers
Lei Yu, DUAN Ji-hua, HUANG Fei-yi, Kang Yan-kai, Luo yi, Chen yu-hong, Ding Ding, Yao Li-na, Dong Li-juan, Li Saijun
(Tea Research Institute, Hunan Academy of Agriculture Sciences/ National Center for Tea Improvement, Hunan Branch/Hunan Observation and Experiment Station of Tea Plant and Tea Processing/Ministry of Agriculture, Hunan Engineering Technology Research Center for Tea variety and seedling)
Abstract:
EST-SSR molecular markers were used to identify the hybrids of two F1 crosses, which was derived from the variety ‘Fuding Dabaicha’ and ‘Anhui 1’ as the female parent and ‘Baojing Huangjincha 1’ as the male parent, followed by a genetic diversity. The results showed that 41 (85.42%) of 48 progenies from Fuding Dabaicha were identified to be true F1 hybrids, while among 35 strains (79.55%) of 44 plants from Anhui 1 were true hybrids. Genetic analysis using 17 pairs of SSR primers revealed 41 alleles in the two hybrid populations with an average of 2.82 (Fuding Dabaicha) and 2.71 (Anhui 1). No significant difference on the genetic diversity parameters of the two hybrid F1 populations were detected. The mean values of Nei, He, Ho, I were 0.56, 0.78, 0.43, 0.93 (Fuding Dabaicha) and 0.55, 0.75, 0.43, 0.90 (Anhui 1), respectively. The variation range of genetic similarity coefficient in F1 hybrid of Fuding Dabaicha × Baojing Huangjincha 1 and Anhui 1 × Baojing Huangjincha 1 was between 0.44~0.90 and 0.48~0.95 respectively. The cluster analysis using UPGMA showed that two hybrid populations were divided into three groups when the similarity coefficient was 0.60 and 0.64 respectively. The progenies were clustered together and represented a higher genetic similarity with respective female parents than the male parent. Collectively, this study reported an example on the genetic classification of tea hybrids using EST-SSR markers.
Key words:  Tea  SSR  hybrid identification  genetic diversity

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