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  • 胡国峰,倪照君,Daouda Coulibaly,等.梅品种S基因型分析及新S基因鉴定[J].植物遗传资源学报,2021,22(3):860-872.    [点击复制]
  • HU Guo-feng,NI Zhao-jun,Daouda COULIBALY,et al.Identification of S Genotype and Novel S-RNase Genes of Eleven Cultivars in Japanese apricot (Prunus mume Sieb. et Zucc.)[J].植物遗传资源学报,2021,22(3):860-872.   [点击复制]
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梅品种S基因型分析及新S基因鉴定
胡国峰, 倪照君, Daouda Coulibaly, 高志红
0
(南京农业大学园艺学院)
摘要:
自交不亲和是植物避免近亲繁殖保持优良种性的重要途径,梅是典型的配子体(GSI)自交不亲和植物。本研究采用AS-PCR(Allele-specific PCR,等位基因特异性PCR)和序列测定等技术,分别以梅S-RNase基因与SFB基因核苷酸序列保守区设计引物Pru-C2和PCE-R、SFB-C1F和Pm-Vb,然后以11个品种的基因组DNA为模板进行AS-PCR扩增和PCR产物测序。经过序列对比分析明确了11个梅品种的S-RNase基因型与SFB基因型,分别为‘软条红梅’(S14S13)(F-box2/SFB14)、‘小叶猪肝’(S3S33)(SFB51/SFB53)、‘中红’(S3S24)(SFB2/SFB24)、‘青佳915’(S3S15)(SFB2/SFB43)、‘美林红’(S1S37)(SFB1/SFB24)、‘赤凤红梅’(S14S38)(SFB12/SFB49)、‘大龙梅’(S20S30)(SFB7/SFB41)、‘红光梅’(S3S33)(SFB24/SFB55)、‘南农丰羽’(S3S5)(SFB31/SFB43)、‘南农丰娇’(S3S6)(SFB2/SFB47)、‘南农龙霞’(S15S37)(SFB41/SFB43)。对获得的S-RNase基因与SFB基因序列进一步分析发现除了已知的S-RNase基因与SFB基因外,还鉴定到S37-RNase,S38-RNase 2个未登录的新S-RNase基因与SFB47、SFB49、SFB51、SFB53、SFB555个未登录的新SFB基因,并将其序列登录在GenBank数据库,其登录号分别为MT950061、MT950062,以及MW186463、MW186465、MW186467、MW186469、MW186471。研究结果为梅生产栽培、授粉树的配置和提高育种效率提供了依据。
关键词:    S-RNase基因型  SFB基因  AS-PCR  新基因
DOI:10.13430/j.cnki.jpgr.20200922002
投稿时间:2020-09-22修订日期:2021-03-17
基金项目:国家重点研发计划 (2018YFD1000107);农业部农作物种质资源保护项目;中央高校基本业务费(KYZZ201911);“一带一路”创新合作项目:园艺作物分子育种技术的海外应用合作开发( BZ2019012)
Identification of S Genotype and Novel S-RNase Genes of Eleven Cultivars in Japanese apricot (Prunus mume Sieb. et Zucc.)
HU Guo-feng, NI Zhao-jun, Daouda COULIBALY, GAO Zhi-hong
(College of Horticulture, Nanjing Agricultural University, Nanjing, 210095)
Abstract:
Self-incompatibility is an important way for plants to avoid inbreeding and thus to maintain excellent species. Japanese apricot is a kind of typical gametophyte self-incompatibility (GSI) species. In this study, AS-PCR (Allele Specific PCR) technique and sequencing were used to analyze and identify S-RNase genotypes and SFB genotypes of 11 cultivars. First, primer pairs Pru-C2 and PCE-R, SFB-C1F and Pm-Vb were designed according to the conservative region of the S-RNase gene and SFB gene of Japanese apricot. Then, the genomic DNA was used as a template for AS-PCR amplification and PCR products sequenced directly. After sequences comparison and analysis, the S-RNase genotype and SFB genotype of 11 Japanese apricot cultivars were ‘Ruantiaohongmei’ of S13S14 and F-box2/SFB14, ‘Xiaoyezhugan’ of S3S33 and SFB51/SFB53, ‘Zhonghong’ of S3S24 and SFB2/SFB24, ‘Qingjia915’ of S3S15 and SFB2/SFB43, ‘Meilinhong’ of S1S37 and SFB1/SFB24, ‘Chifenghongmei’ of S14S38 and SFB12/SFB49, ‘Dalongmei’of S20S30 and SFB7/SFB41, ‘Hongguangmei’ of S3S33 and SFB24/SFB55, ‘Nannongfengyu’ of S3S5 and SFB31/SFB43, ‘Nannongfengjiao’ of S3S6 and SFB2/SFB47, ‘Nannonglongxia’ of S15S37 and SFB41/SFB43. Further analysis of the obtained S-RNase gene and SFB gene sequence showed that in addition to the known S-RNase gene and SFB gene, and two unregistered S-RNase genes of S37-RNase and S38-RNase, and five unregistered new SFB genes SFB47, SFB49, SFB51, SFB53 and SFB55 were also identified. Their sequences have been registered in the GenBank database, the accession numbers were MT950061 and MT950062, MW186463, MW186465, MW186467, MW186469 and MW186471, respectively. The research results provide a basis for the allocation of pollination trees for Japanese apricot production and cultivation and the improvement of breeding efficiency.
Key words:  Prunus mume  S-RNase gene  SFB gene  AS-PCR  Novel gene

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