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  • 金平,朱志玉,张靓,等.温州芜菁与水果紫苤蓝杂交创制芸薹属异源四倍体杂种[J].植物遗传资源学报,2021,22(3):851-859.    [点击复制]
  • PING Jin,ZHU Zhi-yu,ZHANG Liang,et al.Production of Synthesized Brassica Allotetraploids between Wenzhou Turnip And Purple Kohlrabi[J].植物遗传资源学报,2021,22(3):851-859.   [点击复制]
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温州芜菁与水果紫苤蓝杂交创制芸薹属异源四倍体杂种
金平1, 朱志玉1 张靓1, 傅敏杰1, 郑文1, 张萼1, 陈加红2, 吴建国1, 朱祝军1
0
(1浙江农林大学农业与食品科学学院,浙江省农产品品质改良技术研究重点实验室, 杭州 311300; 2杭州万向职业技术学院健康与现代农业系,杭州 310023)
摘要:
本研究以‘温州芜菁’(Brassica rapa L. em. Matzg.,2n=20,AA)为母本,‘水果紫苤蓝’(B. oleracea L. var. caulorapa DC,2n=18,CC)为父本进行远缘杂交,创造二倍体远缘杂种。通过花期剥蕾授粉、子房培养共获得2株杂种,经扩繁得到无性苗19株,经形态学分析、细胞学观察和RAPD分子鉴定出两株均为温州芜菁×紫苤蓝的真杂种,后利用染色体加倍获得异源四倍体新型芜菁甘蓝新种质材料(AACC,2n=38)。两杂种株系出现茎色分离,根部与茎部均有膨大,但程度低于亲本。异源四倍体的花器官较大,花药发育正常,可育花粉率高。本研究所获得的异源四倍体芜菁甘蓝新种质,在一定程度上拓展了芜菁甘蓝种质资源的遗传背景,为研究芜菁甘蓝的起源与进化、遗传改良、生产和推广奠定基础。
关键词:  温州盘菜  水果紫苤蓝  芜菁甘蓝  种间杂种  异源四倍体
DOI:10.13430/j.cnki.jpgr. 20200929004
投稿时间:2020-09-29修订日期:2021-01-17
基金项目:“浙江省省级现代种业发展工程”农作物种质保护项目(2015004);浙江省“三农六方”项目(20405210244)
Production of Synthesized Brassica Allotetraploids between Wenzhou Turnip And Purple Kohlrabi
PING Jin1, ZHU Zhi-yu1, ZHANG Liang1, FU Min-jie1, ZHENG Wen1, ZHANG E1, CHEN Jia-hong2, WU Jianguo1, ZHU Zhujun1
(1Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300; 2Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic,Hangzhou 310023)
Abstract:
In the present study, wide hybridization was performed between ‘Whenzhou turnip’ (Brassica rapa L. em. Matzg.,2n=20, AA) and ‘purple kohlrabi’ (B. oleracea L. var. caulorapa DC, 2n=18, CC) by hand emasculation following tissue culture for hybrid seeds. A total of 2 hybrids were obtained through bud pollination and ovary culture, and 19 asexual seedlings were obtained through expanding propagation. Morphological analysis, cytological observation and RAPD molecular analysis identified the two strains as true hybrids of ‘Wenzhou turnip’ × ‘purple kohlrabi’, and then the novel allotetraploid rutabagas germplasm was obtained by chromosome doubling (AACC, 2n=38). The two hybrid lines were separated by stem color, and their root and stem were enlarged, but the degree was lower than that of the parents. The floral organs of allotetraploid were larger, anthers developed normally, and the pollen viabilities was higher. The new germplasm of allotetraploid rutabagas was obtained in present studies, which expanded the genetic background of rutabagas germplasm resources to a certain extent and laid a foundation for the research on the origin and evolution, genetic improvement, production and promotion of rutabagas.
Key words:  Wenzhou turnip  purple kohlrabi  rutabagas  interspecific hybrids  allotetraploid

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