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  • 李群,王栋,张文兰,等.基于SSR标记的世界豌豆种质遗传多样性分析[J].植物遗传资源学报,2021,22(3):684-691.    [点击复制]
  • LI Qun,WANG Dong,ZHANG Wen-lan,et al.Genetic Diversity Analysis of Pea Germplasm Resources of the World by SSR Markers[J].植物遗传资源学报,2021,22(3):684-691.   [点击复制]
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基于SSR标记的世界豌豆种质遗传多样性分析
李群, 王栋, 张文兰, 田茜, 戴双, 颜廷进
0
(山东省农作物种质资源中心)
摘要:
为综合评价国内外豌豆种质资源及优异基因挖掘利用提供依据,本研究以国内外288份豌豆种质资源为实验材料,利用SSR标记对其进行遗传多样性分析,结果表明,利用筛选出的24对多态性SSR引物,共扩增出153个等位基因,平均每对引物扩增出6.38个等位基因,其中有效等位基因占37.48%,Shannon指数平均为0.9432,参试引物的多态性信息量(PIC)平均为0.4331。亚洲豌豆种质资源遗传变异最为丰富,其基因多样性指数为0.4638,而非洲的豌豆资源遗传基础相对狭窄,其基因多样性指数为0.3480。通过洲际间的聚类分析显示,亚洲、欧洲、美洲、大洋洲、非洲及俄罗斯联邦豌豆种质资源群体之间具有明显的地域分布规律,可分为2个组群,在组群I中又分出2个亚组群,其中欧洲与美洲间遗传距离最近,为0.922,其次是亚洲与非洲的遗传距离为1.425,大洋洲与其他大洲的遗传距离最远,为2.958。这些遗传多样性较高的豌豆种质资源可为我国今后豌豆育种及品种改良提供丰富的遗传材料。
关键词:  豌豆  种质资源  SSR标记  遗传多样性
DOI:DOI:10.13430/j.cnki.jpgr.2020102200
投稿时间:2020-10-22修订日期:2020-12-07
基金项目:山东省农业科学院农业科技创新工程新品种测试与检测公共服务平台(CXGC2017A02);山东省现代农业生产技术体系杂粮产业创新团队建设项目(SDARS-16-01);山东省自然科学基金项目(ZR2017PCM02)
Genetic Diversity Analysis of Pea Germplasm Resources of the World by SSR Markers
LI Qun, WANG Dong, ZHANG Wen-lan, TIAN Qian, DAI Shuang, YAN Ting-jin
(Shandong Center of Crop Germplasm Resources)
Abstract:
In this study, the genetic diversity of 288 domestic and foreign pea germplasm resources were analyzed by SSR technique, which provided a basis for comprehensive evaluation of domestic and foreign pea germplasm resources and the utilization of elite genes. The results showed that 153 alleles were amplified by 24 pairs of SSR primers, with an average of 6.38 alleles per primer. Among them, the effective alleles accounted for 37.48%, the Shannon’s index averaged 0.9432, and the polymorphism information content (PIC) of the tested primers was 0.4331. The genetic diversity index of Asian pea germplasm was 0.4638, while that of Africa was 0.3480. This indicated that the genetic variation of pea germplasm resources in Asia was the most abundant, while that in Africa was relatively narrow. The intercontinental cluster analysis showed that there were obvious regional distribution rules among the pea germplasm resources in Asia, Europe, America, Oceania, Africa and the Russian Federation, which could be divided into two groups. In group I, there were two subgroups. The genetic distance between Europe and America was the closest (0.922), followed by Asia and Africa (1.425), and the genetic distance between Oceania and other continents was the longest at 2.958. These pea germplasm resources with high genetic diversity could provide rich genetic materials for breeding and cultivar improvement.
Key words:  pea  germplasm resources  SSR  genetic diversity

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