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  • 张颖,赵国龙,林春晶,等.大豆核不育基因ms6功能性分子标记的开发与验证[J].植物遗传资源学报,2021,22(3):807-814.    [点击复制]
  • ,et al.Development and Validation of Functional Molecular Markers of Nuclear Sterility Gene ms6 in Soybean[J].植物遗传资源学报,2021,22(3):807-814.   [点击复制]
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大豆核不育基因ms6功能性分子标记的开发与验证
张颖1, 赵国龙2, 林春晶2, 贾顺耕1, 郭凤兰1, 赵丽梅2, 张春宝2
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(1.吉林农业大学农学院,吉林省农业科学院大豆研究所;2.吉林省农业科学院大豆研究所)
摘要:
杂种优势利用是提高作物产量的有效手段之一。随着第三代“智能不育杂交育种技术”的兴起,核不育基因被广泛应用于作物杂交育种中。在大豆中,ms6作为重要的核不育基因被广泛应用于轮回选择育种,但在杂交育种中鲜有报道。本研究利用大豆ms6基因上的突变位点开发了12个dCAPS标记进行分析验证,其中3个dCAPS标记ms6-dCAPS-1、ms6-dCAPS-2和ms6-dCAPS-3的扩增产物酶切后出现明显多态性条带。经进一步验证,明确ms6-dCAPS-3标记稳定且重复性好,其扩增产物经限制性内切酶Hind III酶切后,可将ms6后代分离群体中纯合可育、杂合可育和纯合不育单株准确区分。为ms6核不育基因在杂交大豆育种中的应用提供了有效的技术支撑。
关键词:  大豆  隐性核不育  dCAPS标记  多态性  筛选
DOI:10.13430/j.cnki.jpgr.20201023001
投稿时间:2020-10-23修订日期:2020-11-28
基金项目:吉林省科技发展计划项目(20190101007JH);吉林省农业科技创新工程(c92070422)
Development and Validation of Functional Molecular Markers of Nuclear Sterility Gene ms6 in Soybean
Abstract:
Heterosis utilization is one of the effective means to raise the crop yield. With the rise of the third generation of "intelligent sterility hybrid breeding technology", nuclear sterility gene has been widely used in crop cross breeding. In soybean, ms6 as an important gene of nuclear sterility has been widely used in recurrent selection breeding, but it is rarely reported in hybridization breeding. In this study, 12 dCAPS markers were developed using SNP mutation site of soybean ms6 gene. Three dCAPS markers ms6-dCAPS-1, ms6-CAPS-2 and ms6-CAPS-3 were identified by restriction endonuclease digestion. Further verification showed that the ms6-dCAPS-3 marker was stable and reproducible. After restriction endonuclease Hind III digestion, the amplified products could accurately distinguish homozygous fertile, heterozygous fertile and homozygous sterile single strains in the isolated population of ms6 offspring. This provides an effective technical support for the application of nuclear sterile gene ms6 in hybrid soybean breeding.
Key words:  Glycine max  recessive genic male sterility  dCAPS marker  polymorphism  screening

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