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  • 李永波,崔德周,黄琛,等.细胞自噬提高小麦抗旱性研究[J].植物遗传资源学报,2021,22(3):616-624.    [点击复制]
  • LI Yong-bo,CUI De-zhou,HUANG Chen,et al.Study on Cell Autophagy Increasing Drought Resistance in Wheat[J].植物遗传资源学报,2021,22(3):616-624.   [点击复制]
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细胞自噬提高小麦抗旱性研究
李永波1,崔德周1,黄琛1,隋新霞1,樊庆琦1,楚秀生1,2
0
(1山东省农业科学院作物研究所/黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室,济南250100;2山东师范大学生命科学学院,济南250014)
摘要:
干旱胁迫可引起植物细胞发生自噬,而植物可通过自噬将体内的一些有害物质清除掉,进而提高自身的抗旱性。本研究以抗旱小麦(Triticum aestivum L.)品种平麦 189 号和洛旱 6 号以及干旱敏感小麦品种临麦 2 号和山农优麦 3 号为材料,利用溶酶体荧光探针、Western blot、透射电镜等分子生物学技术,发现抗旱小麦干旱胁迫时自噬相关基因 6 和 8(ATG6、ATG8,autophagy related gene6/8)的表达量、ATG8-PE(磷脂酰乙醇胺)蛋白质的形成以及自噬泡的数量缓慢上升;而在干旱敏感小麦中却表现出急剧上升后下降的趋势。抗旱小麦品种细胞自噬缓慢应答干旱胁迫,且持续时间较长,使幼苗萎蔫迟缓;而干旱敏感小麦品种细胞自噬迅速应答干旱胁迫,但持续时间较短,使幼苗萎蔫加快。本研究为深入阐释小麦抗旱分子机理,筛选抗旱小麦种质资源以及培育抗旱小麦新品种提供了理论基础。
关键词:  小麦  自噬  干旱胁迫  自噬相关基因
DOI:10.13430/j.cnki.jpgr.20201024001
投稿时间:2020-10-24修订日期:2021-01-27
基金项目:国家自然科学基金青年项目(32001542);山东省自然科学基金青年项目(ZR2020QC114);小麦玉米国家工程实验室开放课题 (2018LYZWS06);作物生物学国家重点实验室开放课题基金(2018KF01)
Study on Cell Autophagy Increasing Drought Resistance in Wheat
LI Yong-bo1, CUI De-zhou 1, HUANG Chen1, SUI Xin-xia1, FAN Qing-qi1, CHU Xiu-sheng1,2
(1Crop Research Institute, Shandong Academy of Agricultural Sciences / Key Laboratory of Wheat Biology and Genetic Improvement in Northern Huanghe-Huaihe River Valley / National Engineering Laboratory for Wheat and Maize, Jinan 250100; 2School of Life Science, Shandong Normal University, Jinan 250014)
Abstract:
Drought stress can induce autophagy in plant cells,and plants can remove some harmful substances through autophagy,with the result of increasing their drought resistance. In this study,droughtresistant wheat cultivars Pingmai 189 and Luohan 6 Hao,and drought-vulnerable wheat cultivars Linmai 2 Hao and Shannong Youmai 3 Hao were used as materials. It was found that under drought stress the expression of autophagy-related genes 6 and 8(ATG6,ATG8,autophagy related gene 6/8),the formation of ATG8-PE(phosphatidylethanolamine)and the number of autophagic vacuoles increased slowly in the drought-tolerant cultivars,but increased sharply first and then decreased in the drought-vulnerable cultivars,as detected through the use of lysosome fluorescence probe,Western blot,transmission electron microscope and other molecular biological techniques. The slow yet long-lasting autophagy response of the drought resistant wheat to drought stress slowed down the speed of the seedling wilting,while the fast yet short lasting autophagy response of the drought-vulnerable wheat to drought stress speeded up the seedling wilting. This study provides a theoretical basis for further elucidating the molecular mechanism of drought resistance,screening drought-resistant wheat germplasm resources and breeding new drought-resistant wheat
Key words:  wheat  autophagy  drought stress  autophagy related genes

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