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  • 胡文静,裔新,高德荣,等.小麦品种扬麦13粒重QTL定位[J].植物遗传资源学报,2021,22(3):782-788.    [点击复制]
  • HU Wen-jing,YI Xin,GAO De-rong,et al.Genetic Mapping of the Quantitative Trait Locus Contributes to the Grain Weight in Cultivar Yangmai13[J].植物遗传资源学报,2021,22(3):782-788.   [点击复制]
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小麦品种扬麦13粒重QTL定位
胡文静1, 裔新2, 高德荣1, 朱冬梅1, 陆成彬1, 程顺和1, 张勇1
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(1.江苏里下河地区农业科学研究所/农业部长江中下游小麦生物学与遗传育种重点实验室;2.淮阴师范学院/江苏省环洪泽湖生态农业生物技术重点实验室)
摘要:
粒重是决定小麦产量的关键要素,也是产量育种的重要目标。本研究以CIMMYT引进种质人工合成小麦衍生系C615为母本,扬麦13(Yangmai13,简称YM13)为父本,构建重组自交系(RIL,recombinant inbred lines)群体。利用小麦90K SNP芯片并且结合4个环境下亲本和群体的千粒重表型结果,定位粒重相关QTL。共检测到2个与粒重相关的QTL,分别位于1BL和6AL上,QTGW.yaas-1BL仅能在1个环境下检测到,遗传位置在1BL的12.90 cM,表型贡献率为3.07%,加性效应为0.78,增效基因来源于C615。QTGW.yaas-6AL在4个环境中均能检测到,遗传位置在6AL的112.70 cM~116.00 cM区间,表型贡献率为7.63%~10.55%,加性效应为1.46~1.51,增效基因来源于扬麦13。利用已报道的粒重基因相应KASP (Kompetitive allele-specific PCR) 标记检测亲本,C615和扬麦13共同携有6个粒重相关基因的增加粒重的等位变异,另有3个粒重基因的等位变异在亲本间呈现差异,群体定位中未检测到与这3个差异基因位点一致的QTL。本研究挖掘到的新的粒重位点可为进一步揭示扬麦13粒重的遗传基础和培育高产小麦新品种奠定基础。
关键词:  小麦  90K SNP  粒重  QTL  KASP标记  标记辅助育种
DOI:10.13430/j.cnki.jpgr.20201108001
投稿时间:2020-11-08修订日期:2021-01-04
基金项目:国家重点研发计划项目(2017YFD0100801);江苏省自然科学基金项目(BK20171279);国家自然科学基金(32071999);扬州市现代农业项目(YZ2020033)
Genetic Mapping of the Quantitative Trait Locus Contributes to the Grain Weight in Cultivar Yangmai13
HU Wen-jing1, YI Xin2, GAO De-rong1, ZHU Dong-mei1, LU Chen-bin1, CHENG Shun-he1, ZHANG Yong1
(1.Lixiahe Institute of Agriculture Sciences / Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Yangzhou;2.Jiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian)
Abstract:
Grain weight (GW) is one of key factors contributing the wheat yield. In wheat, breeding for higher GW becomes an important target. In this study, a population of 198 RILs crossed by C615 and Yangmai13 (YM13) was constructed, followed by genotyping with 90K SNP array and quantifying thousand grain weight at four environmental conditions. By using the genome-wide association scanning (GWAS), QTGW.yaas-1BL derived from C615 was detected in one environment at 12.90 cM on 1BL, which accounted for 3.07% of the phenotypic variances and 0.78 of the additive effect. The second locus QTGW.yaas-6AL derived from Yangmai13 was verified at four environments between 112.70 cM~116.00 cM on 6AL, accounting for 7.63%~10.55% of the phenotypic variances and 1.46~1.51 of the additive effect. Genotyping with functional KASP (Kompetitive allele-specific PCR) markers associating with the grain weight revealed six higher grain weight alleles commonly detected in C615 and YM13, and three alleles that are polymorphic between parents. Collectively, the new grain weight QTL identified will lay the foundation for the genetic studies of grain weight in YM13 and in breeding for high-yielding wheat cultivars.
Key words:  Wheat  90K SNP  Grain weight  QTL  KASP markers  Marker-assisted selection

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