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  • 耿君佑,陈建辉,董中东,等.小麦芒性基因的定位与候选基因分析[J].植物遗传资源学报,2021,22(4):1090-1098.    [点击复制]
  • GENG Jun-you,CHEN Jian-hui,DONG Zhong-dong,et al.Mapping and Candidate Gene Analysis of Awn Type in Common Wheat[J].植物遗传资源学报,2021,22(4):1090-1098.   [点击复制]
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小麦芒性基因的定位与候选基因分析
耿君佑, 陈建辉, 董中东, 任妍, 张宁, 孙丛苇, 陈锋, 赵磊
0
(河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室)
摘要:
芒是小麦重要的穗部器官之一,在小麦的光合作用、抗虫、防鸟和产量形成中都起着重要的作用。但是麦芒形成的分子机制仍不是十分清晰。本研究利用Wheat 90K SNP芯片对206份来自于黄淮海地区的小麦材料进行全基因组关联分析,结果显示检测到的与小麦芒性显著关联的SNP位点主要分布于1A、2A、2B、5A和7A染色体上,但显著性较高的位点集中在5A染色体上。同时借助Wheat 660K SNP芯片对一个重组自交系群体进行BSA分析,共检测到500个差异SNP,其中有158个位于5A染色体上,且主要集中在696.6~706.6 Mb区间。结合两种分析方法结果,并根据中国春参考基因组注释最终确定候选基因为前人所报道的B1位点,将其命名为Tipped1;进一步对该基因进行克隆和多态性分析,结果表明小麦芒的调控可能是由位于Tipped1基因启动子区-346 bp处25 bp的插入/缺失多态性和-284 bp处的连锁型SNP多态性共同调控的。本研究能够为解析小麦芒发育的遗传机制和分子育种提供重要参考信息。
关键词:  小麦    Tipped1  基因克隆  多态性分析
DOI:10.13430/j.cnki.jpgr. 20201203002
投稿时间:2020-12-03修订日期:2021-03-24
基金项目:国家自然科学基金项目(31971947);河南省科技攻关项目(212102110246)
Mapping and Candidate Gene Analysis of Awn Type in Common Wheat
GENG Jun-you, CHEN Jian-hui, DONG Zhong-dong, REN Yan, ZHANG Ning, SUN Cong-wei, CHEN Feng, ZHAO Lei
(College of Agronomy / National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University)
Abstract:
Awn in common wheat is one of the important spike organs and plays a critical role in photosynthesis, insect resistance, bird-preventing and yield formation. The genetic basis on its formation and development remains largely unclear. To identify novel genetic loci involved in awn development, a genome-wide association study (GWAS) was performed using a diversity panel of 206 wheat accessions from Huang Huaihai region based on a Wheat 90K SNP array. The results showed that SNPs associated with phenotypic variation were detected on chromosomes 1A, 2A, 2B, 5A and 7A. SNPs with the highest significance were mainly located on chromosome 5A. Moreover, the bulked segregant analysis (BSA) in a recombinant inbred line population were analyzed using Wheat 660K SNP array. Out of 500 polymorphic SNPs, 158 were found in the 696.6-706.6 Mb interval of chromosome 5A, where the previously reported B1 locus encoding for Tipped1 was resided on the gene annotation of Chinese Spring. Gained from the re-sequencing result of this gene in parental lines, the wheat awn may be associated with a 25-bp insertion/deletion polymorphism located at -346 bp and a linkage SNP at -284 bp in the promoter region. Collectively, this study provided information for understanding the genetic mechanism of wheat awn development and molecular breeding.
Key words:  common wheat  awn  Tipped1  gene cloning  polymorphism analysis

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