引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

过刊浏览    高级检索

本文已被:浏览 448次   下载 0  
不同来源特种稻香味和黑色种皮基因的鉴定与遗传特性分析
曾跃辉, 韦新宇, 黄建鸿, 肖长春, 张锐, 尚伟, 许旭明
0
(三明市农业科学研究院)
摘要:
特种稻是一类具有特殊遗传性状和用途的水稻品种,目前已成为杂交水稻育种的一个重要方向,选择和利用不同来源特种稻种质资源并对其特殊性状基因型进行鉴定和遗传特性分析,对进一步促进特种稻的遗传改良及新品种的选育具有重要意义。本研究利用特异性分子标记InDel-E2、FMbadh2-E7、CAPSRa和CAPS-Ra以及自主开发设计的特异性功能标记FMbadh2-E2、FMbadh2-E7A、FMbadh2-E7B、Ra-CAPS1和Ra-CAPS2并结合基因克隆和测序技术对来自不同地区的32份优质香型水稻亲本材料的香味基因和8份黑米水稻品种的黑色种皮基因进行了基因型鉴定和分析。研究发现,在32份香型水稻材料中,有28份香味性状来源于水稻第8号染色体上编码甜菜碱脱氢酶基因Badh2的功能缺失突变,且为常见的badh2-E7等位突变类型,其在Badh2基因第7外显子处发生8-bp缺失和3个单核苷酸(SNPs)位点多态性突变;2份(板仓香糯和八桂香)表现为香味基因杂合型(Badh2/badh2);2份(溪香和莉香占)在Badh2基因第2和第7外显子处均未发生碱基缺失突变,且具有完整的Badh2基因位点编码序列,其香味性状可能不受Badh2等位基因控制。另外,研究发现,8份黑米水稻品种的黑色种皮均由位于水稻第4号染色体上的Ra/Pb基因控制,其在第7外显子处均表现为2-bp(GT)的缺失突变。同时本研究通过构建多个杂交F2群体对Badh2和Ra基因进行了遗传特性分析,发现其香味和黑色种皮性状分别受1对细胞核隐性和1对细胞核显性主效基因控制。本研究通过对不同来源优质香型水稻亲本材料的香味基因和黑米水稻品种的黑色种皮基因进行鉴定、特异性功能分子标记的开发以及遗传特性分析,为进一步通过分子标记辅助选择育种技术,选育优质、高产且具有香味的杂交水稻新品种及黑米品种提供了重要的理论基础,可明显提高特种稻的选育效率,同时在进一步为发现和挖掘新的香味控制基因及黑色种皮基因的研究上具有重要意义。
关键词:  水稻  香味基因  黑色种皮基因  badh2  Ra/Pb  分子标记
DOI:10.13430/j.cnki.jpgr.20201218001
投稿时间:2020-12-18修订日期:2021-03-22
基金项目:国家水稻产业技术体系专项(CARS-01);福建省自然科学(2018J01141);三明市科技计划项目(2019-N-4)
Identification and Genetic Analysis of the Genes for Fragrance and Black Pericarp in Special Rice from Different Regions
ZENG Yue-hui, WEI Xin-yu, HUANG Jian-hong, XIAO Chang-chun, ZHANG Rui, SHANG Wei, XU Xu-ming
(Sanming Academy of Agricultural Science)
Abstract:
Generation of new special rice varieties with particular inherited characteristics are one of the important targets in hybrid rice breeding. The selection and utilization of special rice germplasm resources derived from different regions and identification of their genotypes and analysis of their particular characteristics are of great significance for further promoting the genetic improvement. By using functional molecular markers InDel-E2, FMbadh2-E7, CAPSRa, CAPS-Ra, FMbadh2-E2, FMbadh2-E7A, FMbadh2-E7B, Ra-CAPS1 and Ra-CAPS2, the last 5 of which are newly designed in this study, combined with gene cloning and sequencing technology, we identified the fragrance genotypes of 32 high-quality fragrant rice materials and the black pericarp genotypes of 8 black rice varieties, respectively, from different regions. Of the 32 fragrant rice varieties, 28 were found to have the fragrance character derived from the known badh2 allele (badh2-E7), which was located on rice chromosome 8 and had an 8-bp deletion and three single nucleotide polymorphisms (SNPs) in exon 7, and which was non-functional for encoding betaine aldehyde dehydrogenase (BADH2), two varieties (Bancangxiangnuo and Baguixiang) were found to be heterozygotic with Badh2/badh2, and the other two (Xixiang and Lixiangzhan) were found to lack the mutation in the coding region of Badh2 including both exon 2 and exon 7, thus without their fragrance character being controlled by Badh2 alleles. In addition, we found that the black pericarp character of all 8 black rice varieties was controlled by the Ra/Pb gene on rice chromosome 4, which has a 2-bp (GT) deletion in exon 7. Moreover, we analyzed the genetic characteristics of Badh2 and Ra genes by developing a number of F2 populations, and found that the fragrance and black pericarp were controlled by a nuclear recessive gene and a nuclear dominant gene, respectively. In this study, the identification and genetic analysis of fragrance gene in 32 high-quality fragrant rice materials and black pericarp gene in 8 black rice varieties derived from the different regions have been made, which provides an important theoretical foundation for further breeding of fragrant hybrid rice and black hybrid rice with high quality and high yield by molecular marker-assisted selective breeding technology, as well as for improving the efficiency in breeding of special rice, and for making new breakthrough to the discovery of novel fragrance gene and black pericarp gene.
Key words:  rice  fragrance gene  black pericarp gene  badh2  Ra/Pb  molecular marker

用微信扫一扫

用微信扫一扫