引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

过刊浏览    高级检索

本文已被:浏览 417次   下载 0  
水稻叶宽调控基因ZY1的图位克隆与功能分析
王迪, 王 健, 程保山, 李 刚, 高 浩, 徐卫军, 袁彩勇
0
(江苏徐淮地区淮阴农业科学研究所)
摘要:
叶宽是水稻株型的重要组成部分,适宜的叶宽有助于提高光利用效率进而提高水稻产量。利用水稻叶宽突变体,阐明新的水稻叶宽调控相关基因的功能,有助于进一步阐明水稻叶宽发育调控机制。本研究利用育种中间材料中发现的窄叶突变体zhaiye1(zy1),与野生型相比,zy1出现明显的窄叶表型,株高也出现显著降低,穗型减小,种子呈现开颖畸形。组织观察结果说明,zy1中是由于叶脉数量减少导致窄叶表型的出现。通过计算F2群体的表型分离比,发现窄叶表型显示由2个隐性遗传位点控制。图位克隆最终将突变基因分别定位到11染色体短臂顶端76kb和12染色体短臂顶端150kb的区域内,利用测序和PCR扩增验证最终证明突变基因为Os11g01130和Os12g01120,二者为重复基因,编码一个OsWOX3A转录因子。进化树分析表明,ZY1蛋白与疣粒野生稻中的同源蛋白进化关系最近。ZY1蛋白亚细胞定位于细胞核中。转录组分析结果说明ZY1突变后差异表达基因分布在多个信号途径中,同时ZY1可能通过生长素信号途径调控水稻叶宽发育。综上所述,ZY1不仅调控水稻叶宽发育,而且参与水稻株高、穗型、颖壳等多个性状的发育调控。
关键词:  水稻  窄叶  图位克隆  ZY1
DOI:10.13430/j.cnki.jpgr. 20210129001
投稿时间:2021-01-29修订日期:2021-04-06
基金项目:江苏省自然科学基金青年基金(BK201902239),淮安市农业科学研究院高层次引进人才科研启动发展基金项目(0062019016B),淮安市农业科学研究院院长科研基金项目(HNY201702)
Map-based Cloning and Function Analysis of Leaf Width Regulation Gene ZY1 in Rice
WANG Di, WANG Jian, CHENG Bao-shan, Li Gang, Gao Hao, XU Wei-jun, YUAN Cai-yong
(Huaiyin Institute of Agricultural Sciences in Xuhuai Region of Jiangsu, Huai’an)
Abstract:
Leaf width is one of the key determinants of plant architecture, and optimal leaf width is important to improve the light energy utilization efficiency and the yield production in rice. Identification of the new leaf width mutant and elucidation of its function is of interest to reveal the regulation mechanism. This study reported the zhaiye1 (zy1) mutant, which showed narrow leaf, reduced plant height, shortened panicle, opened spikelets and narrow-thin grains if compared with wild type (WT). The narrow leaf phenotype in zy1 is resulted mainly from the decrease in the number of large veins and small veins. Segregation analysis in a F2 population suggested two independent loci modulating the narrow leaf phenotype, which were further allocated to the regions of 76kb on chromosome 11 and 150kb on chromosome 12, respectively. Tow candidate genes Os11g01130 and Os12g01120 are duplicate genes and encode for the OsWOX3A transcriptional activator. The fusion protein stacking ZY1 with a C’-terminal in-frame GFP was localized to the nucleus in rice protoplastsm, while ZY1 was phylogenetically clustered with the homolog of O. meyeriana. In zy1 mutant, the transcriptome analysis revealed abundant genes in multiple signaling pathways (e.g.auxin signaling pathway). Collectively, these results suggested that ZY1 was involved in the development of leaf width as well as the regulation of plant height, panicle type, glume shell and other traits.
Key words:  rice  narrow leaf  map-based cloning  ZY1

用微信扫一扫

用微信扫一扫