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  • 于跃,孙健,张静,等.198份大麻种质资源农艺及品质性状综合评价[J].植物遗传资源学报,2021,22(4):1021-1030.    [点击复制]
  • Yu Y,Sun J,Zhang J,et al.Comprehensive evaluation on agronomic and quality traits of 198 hemp germplasm resources[J].植物遗传资源学报,2021,22(4):1021-1030.   [点击复制]
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198份大麻种质资源农艺及品质性状综合评价
于跃1, 孙健1, 张静1, 杨泽茂1, 张利国2, 邓灿辉1, 程超华1, 唐蜻1, 许英1, 陈小军1, 张小雨1, 戴志刚1, 粟建光1
0
(1.中国农业科学院麻类研究所/农业部麻类生物学与加工重点实验室;2.黑龙江省农业科学院经济作物研究所)
摘要:
本研究以198份国内外大麻种质资源为材料,开展了14个农艺和品质性状多样性鉴定,并进行变异分析、相关性分析、主成分分析和聚类分析的综合评价,为我国大麻种质资源创新和资源高效利用提供基础材料和技术参考。结果表明,198份国内外大麻种质资源具有较为丰富的遗传多样性,14个性状的变异系数范围为4.79%~64.45%,变异系数最大的是CBD含量,最小的是雌花开花日数,平均值为27.78%,大麻酚(CBN)、大麻二酚(CBD)、大麻二酚酸(CBDA)、次大麻二酚(CBDV)4种大麻素含量的变异系数均大于40.0%,品质性状表现出更好的变异性;相关性分析表明,CBD含量与CBDA、CBDV和CBN的含量均为极显著正相关,CBDA含量与CBN含量极显著正相关,株高与茎粗极显著正相关,CBDA含量与多个农艺性状呈现显著的相关性,株高、茎粗的改良,有利于大麻素CBDA含量的提高;主成分分析把14个性状综合为6个主成分,累计贡献率达到69.204%,表明6个主成分反映了大麻种质资源大部分的性状信息。聚类分析得到3个类群,其中第Ⅰ类群的69份资源在大麻素含量、株高和茎粗上表现较好,可以进一步筛选作为育种研究的亲本材料。
关键词:  大麻  种质资源  农艺性状  品质性状  评价
DOI:10.13430/j.cnki.jpgr.20210208002
投稿时间:2021-02-08修订日期:2021-03-01
基金项目:现代农业产业技术体系建设专项(CARS-16-E01);农作物种质资源保护与利用专项(2016NWB044);国家科技资源共享服务平台项目(NCGRC-2020-15)
Comprehensive evaluation on agronomic and quality traits of 198 hemp germplasm resources
Yu Y1, Sun J1, Zhang J1, Yang Z M1, Zhang L G2, Deng C H1, Cheng C H1, Tang Q1, Xu Y1, Chen X J1, Zhang X Y1, Dai Z G1, Su J G1
(1.Institute of bast fiber crops, Chinese Academy of Agricultural Sciences/ Key Laboratory of Biology and Processing of Hemp, Ministry of Agriculture;2.Institute of Cash Crops, Heilongjiang Academy of Agricultural Sciences)
Abstract:
In order to provide germplasm resource and technical reference for the innovation and efficient utilization of cannabis germplasm resources in China, this study analyzed phenotypic variations on 14 agronomic and quality related traits in 198 cannabis germplasm accessions collected from China, Morocco and European countries. The results showed that either domestic or exotic cannabis germplasm accessions represented relatively abundant genetic diversity, with variation coefficients on 14 traits ranged from 4.79%-64.45%. The highest variation coefficient was CBD content and the lowest was female flowering date, with an average value of 27.78%. The variation coefficient regarding to four cannabinoids (CBN, CBD, CBDA and CBDV) is all higher than 40.0%, suggesting better variability at quality traits. The CBD content was found to significantly positively correlate with the content of CBDA, CBDV and CBN; the CBDA content was significantly positively correlated with the CBN content; the plant height was significantly positively correlated with the stem diameter. The CBDA content was significantly correlated with multiple agronomic traits, such as plant height and stem diameter. Principal component analysis revealed six principal components with a cumulative contribution rate of 69.204%, indicating that six principal components are able to represent large proportion of these phenotypic variations. Cluster analysis suggested three groups, in which 69 accessions of the first group showed better perfermance in terms of cannabinoid content, plant height and stem diameter, and they might be further tested for obtaining elite parental lines in breeding.
Key words:  cannabis  germplasm resources  agronomic characters  quality character  evaluation

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