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玉米花丝颜色基因qSC10精细定位及候选基因分析
代资举1, 张莹莹2, 王新涛2, 杨青2, 王艳2, 李保全2
0
(1.河南省作物分子育种研究院;2.河南省农业科学院作物设计中心)
摘要:
为解析玉米花丝颜色性状的遗传机理,挖掘其候选基因及等位变异,本研究以郑58和昌7-2为亲本构建的重组自交系(RIL)群体为材料,对控制玉米花丝颜色QTL进行定位。利用郑58与控制玉米粉色花丝主效位点qSC10的近等基因系qSC10-NIL杂交构建F3作图群体,对主效位点qSC10进行精细定位,根据qSC10定位区间和NCBI数据库中B73 RefGen-v4玉米参考基因组序列预测候选基因。结果表明,利用郑58和昌7-2构建的RIL群体共鉴定出4个控制玉米花色的主效QTL,分别位于第 1、8、9、10染色体上,可解释3.34%~12.28%的表型变异。位于第10染色体上的主效显性位点qSC10精细定位在374.2 Kb区间之内。参考B73基因组序列,在定位区间内包含14个预测基因,其中包括1个影响玉米种皮颜色基因R-S(GRMZM5G822829)。 基因表达分析结果表明R-S基因在花丝、根、茎、叶和雄穗中均有表达,qSC10-NIL花丝中表达量极显著高于郑58,推测该基 因可以正向调控花色素合成相关基因表达,影响花色素在玉米花丝中的积累。
关键词:  玉米  花丝颜色基因  QTL定位  精细定位  候选基因分析
DOI:10.13430/j.cnki.jpgr.20210217001
投稿时间:2021-02-17修订日期:2021-03-05
基金项目:河南省科技攻关项目(202102110155);河南省农业科学院优秀青年基金(2020YQ33)
Fine Mapping and Candidate Gene Analysis of Silk Color Gene qSC10 in Maize (Zea mays L.)
DAI Zi-ju1, ZHANG Ying-ying2, WANG Xin-tao2, YANG Qing2, WANG Yan2, LI Bao-quan2
(1.Institute of Crops Molecular Breeding, Henan Academy of Agricultural Sciences;2.Crop Designing Centre, Henan Academy of Agricultural Sciences)
Abstract:
In order to understand the genetic basis of silk color in maize, the candidate genes and excellent alleles associating with silk color were explored. The recombinant inbred lines (RIL) crossed from maize cultivars “Zheng 58” and “Chang 7-2” were used for QTL mapping. A fine mapping of the major gene qSC10 causing pink silk was conducted in a F3 population of “Zheng 58” that hybridized with a near isogenic line qSC10-NIL (harboring qSC10), followed by a physical mapping and candidate gene analysis according to the reference genome sequence of B73 (RefGen-v4). Four major QTL were identified on chromosomes 1, 8, 9 and 10 using the RIL families, and each explained 3.34% to 12.28% phenotypic variation. The gene qSC10 on chromosome 10 was delimited to a 374.2 kb interval which contains 14 annotated genes, including the R-S gene (GRMZM5G822829) that was associated with seed coat color. The R-S gene was found to be expressed in silk, root, leaf and tassel, while the expression level of qSC10-NIL in silk was significantly higher than that of Zheng 58. Collectively, these results suggested that the R-S gene, which is a candidate gene for qSC10, may contribute to the accumulation of anthocyanins in maize filament by a positive regulation on the expression of anthocyanin synthesis-related genes.
Key words:  maize  silk color gene  QTL mapping  fine mapping  candidate-gene analysis

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