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基于表型性状和SSR分子标记构建甜荞初级核心种质
李金龙, 范昱, 赵梦雨, 康珍, 杨克理, 张凯旋, 周美亮
0
(中国农业科学院作物科学研究所,北京10081)
摘要:
本研究利用各个材料的来源地信息和6个表型性状以及7对SSR标记,对1287份甜荞种质资源的遗传多样性进行了评价。表型性状分析结果表明,6个表型性状在不同的甜荞种质资源中表现出较大的差异,变异系数的变化范围17.13%~33.87%之间,平均为24.25%,其中茎粗的变异系数最大,是造成表型变异的主要因素。表型聚类将所有材料划分为5个亚群,大部分材料集中在第5亚群,其余材料则表现出一定的区域性特征。SSR标记分析表明,1287份材料的相似性系数在0.0477~0.9486,平均值为0.4289。基于SSR分子标记的聚类分析表明,种质资源与其地理分布存在一定的相关性。表型性状和SSR分子标记结果均表明甜荞种质资源具有丰富的遗传多样性,最终构建了530份的甜荞初级核心种质。
关键词:  荞麦  种质资源  农艺性状  遗传多样性
DOI:10.13430/j.cnki.jpgr.20210308001
投稿时间:2021-03-08修订日期:2021-05-11
基金项目:国家重点研发计划(2019YFD1001300, 2019YFD1001302)
Construction of Primary Core Collection of Buckwheat Germplasm Resources Based on Phenotypic Traits and SSR
LI Jin-long, FAN Yu, ZHAO Meng-yu, KANG Zhen, YANG Ke-li, ZHANG Kai-xuan, ZHOU Mei-liang
(Institute of Crop Sciences, Chinese Academy of Agricultural Sciences)
Abstract:
In this study, the genetic diversity of 1287 common buckwheat germplasm resources was evaluated by using the geographical information, 6 phenotypic traits and 7 pairs of SSR markers. The analysis of six phenotypic traits revealed great variation in different germplasm resources. The coefficient of variation ranged from 17.13% to 33.87%, with an average of 24.25%. The coefficient of variation of stem diameter was the largest, which was the main factor causing phenotypic variation. The1287 accessions were divided into five groups, most of which were clustered in the fifth subgroup, and the others showed regional specificity. The analysis of SSR indicated the index of similarity coefficient ranged from 0.0477 to 0.9486 with an average of 0.4289. Clustering analysis based on SSR molecular markers showed that there was a certain correlation between germplasm resources and their geographical distribution. The results of phenotypic traits and SSR molecular markers showed that there was abundant genetic diversity in common buckwheat germplasm resources and 530 accessions of primary core collection were constructed.
Key words:  buckwheat  germplasm resources  agronomic traits  genetic diversity

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