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紫花苜蓿MsFtsH8基因的克隆及功能分析
余倩文1, 李明娜2, 龙瑞才2, 杨青川2, 康俊梅2, 郭长虹1
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(1.哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞与遗传育种重点实验室;2.中国农业科学院北京畜牧兽医研究所)
摘要:
盐胁迫是影响植物生长发育的主要非生物胁迫之一。金属蛋白酶FtsH(Filamentation Temperature-Sensitive H)是一种ATP和Zn2+依赖型蛋白酶,广泛存在于真核生物和原核生物中,属于AAA蛋白酶家族(ATPase associated with various cellular activities),在植物叶绿体发育、光合系统蛋白合成和修复、对非生物胁迫的应答和抵抗、植物叶片杂色的调控等重要植物生长发育过程中起到了重要的作用。FtsH在拟南芥、油菜、大豆等植物中多有研究,但在紫花苜蓿中的功能还尚未有详细报导。本研究克隆且分析了紫花苜蓿MsFtsH8基因的表达模式,并对该基因进行生物信息学分析,通过在拟南芥中过表达发现,过表达的拟南芥植株在盐胁迫条件下,种子发芽率、根长和鲜重显著提高。研究结果表明,MsFtsH8具有调控耐盐的功能。本研究为苜蓿耐盐分子育种提供重要候选基因和理论参考。
关键词:  紫花苜蓿  金属蛋白酶FtsH  盐胁迫  蛋白水解酶
DOI:10.13430/j.cnki.jpgr.20210309001
投稿时间:2021-03-09修订日期:2021-03-19
基金项目:哈尔滨师范大学学术创新项目(HSDSSCX2020-09);中央级公益性科研院所基本科研业务费专项(2020-YWF-YB-05)
Cloning and Functional Analysis of MsFtsH8 Gene from Medicago sativa
YU Qian-wen1, LI Ming-na2, LONG Rui-cai2, YANG Qing-chuan2, KANG Jun-mei2, GUO Chang-hong1
(1.College of Life Science and Technology, Harbin Normal University/ Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province;2.Institute of Animal Science, Chinese Academic of Agriculture Science)
Abstract:
Salt stress is one of the main abiotic stresses affecting plant growth and development. Metalloproteinase FtsH (Filamentation temperature-sensitive H) is a type of ATP and Zn2+ dependent protease in the AAA family of proteases (ATPase associated with various cellular activities), and it plays an important role in plant chloroplast development, photosynthetic system protein synthesis and repair, response and resistance to abiotic stress, leaf noise regulation and other important processes. Although FtsH has been extensively studied in several species such as Arabidopsis thaliana, Brassica napus, Glycine max, its function in Medicago sativa has not been reported. In this study, we cloned and analyzed the expression pattern of MsFtsH8 gene in Medicago sativa, followed by a Bioinformatics analysis of the gene sequence. Over-expression of the gene in A. thaliana significantly increased seed germination rate, root length and fresh weight under salt stress. Collectively, this study provides important candidate genes and theoretical reference for alfalfa molecular breeding regarding to salt tolerance.
Key words:  Medicago sativa  metalloproteinase FtsH  salt stress  proteolytic enzymes

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