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苦荞FtHSFA6b转录因子协同调控耐盐功能
左倩1, 伍小方1, 杨克理2, 肖璐1, 周美亮2
0
(1湖南科技大学生命科学学院,湘潭 411201;2中国农业科学院作物科学研究所,北京 100081)
摘要:
本实验从川荞1号中克隆到FtHSFA6b。基因结构分析表明:FtHSFA6b基因由2个外显子和1个内含子组成,其CDS全长1047bp,编码349个氨基酸。进化关系分析表明:FtHSFA6b蛋白与藜麦和棉花亲缘关系较近。启动子顺式作用元件分析表明:该基因启动子顺式作用元件分为5类。qRT-PCR分析表明:FtHSFA6b基因在根茎叶中均有表达,而在茎中表达量最高;用MeJA和NaCl处理,显示FtHSFA6b基因的表达量活性明显受茉莉酸(Methyl Jasmonate,MeJA)和NaCl诱导。毛状根中总黄酮含量分析表明:FtHSFA6b的过表达可增加毛状根对盐处理的耐受性,其总黄酮含量也明显增加;为了进一步研究FtHSFA6b主要影响毛状根类黄酮合成中的具体途径,选择5中关键酶基因C4H、4CL、FLS、F3’、RT1进行qRT-PCR分析,结果显示过表达FtHSFA6b通过影响类黄酮合成途径中的芦丁合成途径,以提高对盐的耐受性。转基因拟南芥中,FtHSFA6b的过表达通过降低MDA含量、增加CAT和POD的活性,来提高拟南芥萌发过程中对盐处理的耐受性。
关键词:  荞麦  FtHSFA6b  毛状根  拟南芥  抗盐分析
DOI:10.13430/j.cnki.jpgr. 20210316004
投稿时间:2021-03-16修订日期:2021-04-13
基金项目:国家重点研发计划(2019YFD1001300, 2019Y FD1001302)
The Transcription Factor FtHSFA6b of Tartary Buckwheat Synergistic Regulation of Salt Tolerance
Zuo Qian1, Wu Xiao-fang1, Yang Ke-li2, Xiao Lu1, Zhou Mei-liang2
(1College of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201;2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081)
Abstract:
In order to study the function of HSF transcription factor in Tartary buckwheat, a HSF family gene was cloned from ‘Chuanqiao No.1’ in this study. The open reading frame is 1047 bp in length encoding for 349 amino acids. The analysis of gene structure showed that FtHSFA6b gene is composed of two exons and one intron. The sequence alignment and phylogenetic analysis showed that FtHSFA6b protein was closely related to CQA-2C in Chenopodium quinoa and GHA-7A in Gossypium hirsutum. Cis-acting elements in the promoter of FtHSFA6b were predicted and divided into five categories. Furthermore, tissue-specific expression analysis showed that FtHSFA6b gene was expressed in roots and leaves, with the lowest expression in roots and the highest expression in stems. qRT-PCR showed that the expression of FtHSFA6b was induced under MeJA or NaCl treatments. The analysis of total flavonoids content in transgenic hairy roots showed that the overexpression of FtHSFA6b could increase the tolerance of hairy roots to salt treatment, and the total flavonoids content also increased significantly. In transgenic Arabidopsis thaliana, over-expressing FtHSFA6b can improve the salt tolerance at the germination stage, possibly by decreasing malondialdehyde (MDA) content and increasing the activities of Catalase (CAT) and peroxidase (POD).
Key words:  buckwheat  FtHSFA6b  hairy root  Arabidopsis  salt resistance analysis

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