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北方春大豆苗期根系性状优异等位变异的发掘
蒋红娇, 王财金, 赵慧艳, 王 洋
0
(黑龙江大学现代农业与生态环境学院)
摘要:
检测与根系性状关联的位点并发掘其携带的优异等位变异,对改良大豆根系性状有重要意义。本研究选取北方春大豆242份育成品种和102份地方品种作为试验材料,2018和2019年在室内对根系性状进行鉴定,利用175对SSR引物进行连锁不平衡和群体结构分析,并采用GLM进行关联分析。结果显示:无论共线性组合还是非共线性组合群体均存在不同程度的连锁不平衡现象。育成品种由2个亚群组成,两年共同检测到17个位点与6个根系性状关联,表型变异解释率为1.35%~7.83%;地方品种由两个亚群构成,两年共同检测到12个位点与5个根系性状关联,表型变异解释率为3.89% ~16.64%。两类群体中共同检测到的2个位点,分别是与主根长关联位点Satt309和与根平均直径关联位点Sat_358。在育成品种中发掘出的增效优异等位变异共38个,载体材料共29个,其中主根长、总根长、根平均直径、根体积、根表面积、根尖数的增效最大的等位变异及载体材料分别为Satt309-122bp(合丰35)、Sat_272-254bp(黑河16)、Sat_358-236bp(吉林12)、Sat_381-193bp(绥农26)、Sat_381-193bp(垦豆18)、Sat_381-224bp(合丰51)。在地方品种中发掘出的增效优异等位变异共33个,载体材料共31个,其中主根长、总根长、根平均直径、根体积、根表面积、根尖数的增效最大的等位变异及载体材料分别为Satt220-242bp(小白脐)、Satt683-206bp(汪清神仙洞)、Sat_160-174bp(平顶香-2)、Satt239-200bp(临江大黄豆)、Satt247-245bp(白露豆-1)、Satt156-227bp(小白脐)。这些优异等位变异和载体材料可以作为优异亲本配置杂交组合,选育具有优良根系性状的大豆品种。
关键词:  北方春大豆  苗期  根系性状  优异等位变异
DOI:10.13430/j.cnki.jpgr.20210401002
投稿时间:2021-04-01修订日期:2021-07-10
基金项目:农业农村部作物种质资源保护(2018NWB036-12)
Mining of Elite Alleles Controlling Root Traits at the Seedling Stage in North Spring Soybean
JIANG Hong-jiao, WANG Cai-jin, ZHAO Hui-yan, WANG Yang
(College of Modern Agriculture and Ecological Environment, Heilongjiang University)
Abstract:
Genetic improvement of root traits in soybean largely relied on exploring the elite loci that associated with beneficial root traits. In this study, 242 northern spring cultivars and 102 landraces were subjected for analyzing phenotypic variation in root under laboratory conditions in 2018 and 2019. 175 pairs of SSR primers were applied for genotyping, followed by the genetic linkage disequilibrium analysis, the population structure analysis, and association analysis using general linear model (GLM). The results show that both collinear combination and non-collinear combination groups have different degree of linkage disequilibrium. The cultivars were clustered into two subpopulations. Seventeen genetic loci associated with six root traits were detected showing the phenotypic variation ranged from 1.35% to 7.83%. The landraces were assigned into two subgroups. Twelve genetic loci were found to be associated with five root traits, which contributed to 3.89% to 16.64% on phenotypic variation. Two genetic loci Satt309 and Sat_358, which were simultaneously detected in the two cultivar and landrace populations, were associated with main root length and root average diameter, respectively. A total of 38 alleles with positive effect were present in 29 cultivars. Out of them, the alleles of main root length (MRL), root length (RL), root average diameter (RAD), root volume (RV), root surface area (RSA), root tip number (RTN) with the greatest synergistic effect were Satt309-122bp (Hefeng35), Sat_272-254bp (Heihe16), Sat_358-236bp (Jilin12), Sat_381-193bp (Suinong26), Sat_381-193bp (Kendou18), and Sat_381-224bp (Hefeng51), respectively. A total of 33 alleles with synergistic effect were found from 31 landraces. For example, the alleles governing MRL, RL, RAD, RV, RSA, RTN which had the greatest synergistic effects, were Satt220-242bp (Xiaobaiqi), Satt683-206bp (Wangqingshenxiandong), Sat_160-174bp (Pingdingxiang-2), Satt239-200bp (Linjiangdahuangdou), Satt247-245bp (Bailudou-1), and Satt156-227bp (Xiaobaiqi), respectively. Collectively, this study identified the superior alleles and donor lines which could be used in breeding for soybean varieties with superior root traits.
Key words:  North Spring Soybean  seedling stage  root traits  elite alleles

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