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阳桃R2R3-MYB家族成员鉴定及其在木质素合成过程中的表达
赵亚梅1, 陈 蕾1, 吴春梅1, 秦 思1, 翟俊文1, 任惠2, 吴沙沙1
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(1.福建农林大学园林学院/兰科植物保护与利用国家林业和草原局重点实验室;2.广西壮族自治区农业科学院园艺研究所)
摘要:
MYB家族已被证实在植物生长发育、类黄酮合成、环境胁迫、木质素生物合成等多个生理方面发挥着重要的作用。为探究阳桃MYB家族在木质素生物合成过程中的作用,本研究基于全基因组数据,对阳桃(Averrhoa carambola)R2R3-MYB转录因子进行了全面的生物信息学分析,并采用RT-qPCR技术,对8个阳桃R2R3-MYB基因在阳桃小枝(T1、T3、T6)、果和叶3个组织不同时期的表达情况进行了验证。结果显示,阳桃基因组中共包含57个1R-MYB、100个R2R3-MYB、4个3R-MYB,100个阳桃R2R3-MYB(AcMYBs)基因不均匀的分布在11条染色体上,被分为24个亚组,相同亚组的基因具有相似但不完全相同的基因结构和保守基序。片段重复和串联重复在阳桃MYB家族的扩张中发挥了重要的作用,且重复产生的基因都经历了纯化选择。同时,阳桃R2R3-MYB家族与拟南芥(Arabidopsis thaliana)及毛果杨(Populus trichocarpa)都具有较强的共线性关系。RT-qPCR结果表明,除AcMYB05外,AcMYB41、AcMYB65、AcMYB84、AcMYB87、AcMYB92、AcMYB97和AcMYB100都在T3和T6时期呈现出较高的表达,在T1和果、叶中几乎不表达。本研究表明,7个AcMYBs的表达具有明显组织特异性,其可能参与到阳桃木质素生物合成途径,为进一步研究MYB家族对木质素生物合成的影响提供了重要的参考价值。
关键词:  阳桃  木质素  R2R3-MYB转录因子  全基因组鉴定  表达模式
DOI:10.13430/j.cnki.jpgr.20210412002
投稿时间:2021-04-12修订日期:2021-04-22
基金项目:广西科技重大专项(桂科AA17204045-4);广西农业科学院基本科研业务专项(桂农科2021YT046);福建农林大学艺术学院、园林学院(合署)学科专业建设项目(YSYL-bdpy-1)
Identification of the R2R3-MYB Family Members in Averrhoa carambola and Their Expression Involved in Lignin Biosynthesis
ZHAO Ya-mei1, CHEN Lei1, WU Chun-mei1, QIN Si1, ZHAI Jun-wen1, REN Hui2, WU Sha-sha1
(1.Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization /College of Landscape Architecture, Fujian Agriculture and Forestry University;2.Horticulture Research Insistute of Guangxi Academy of Agricultural Science)
Abstract:
MYB family transcription factors have been proved to play an important role in biological process, such as plant growth and development, flavonoid synthesis, environmental stress, lignin biosynthesis. To explore if the MYB family members are involved in the lignin biosynthesis in Averrhoa carambola, a genome-wide bioinformatics analysis of R2R3-MYB transcription factor has been conducted, followed by the RT-qPCR analysis to valid the expression levels of the eight R2R3-MYB genes in twigs (T1, T3, T6), fruits and leaves at different stages. Fifty-seven 1R-MYB, 100 R2R3-MYB and four 3R-MYB have been identified. Out of them, 100 R2R3-MYB (AcMYBs) were detected to be unevenly distributed on 11 chromosomes and they were divided into 24 subclades (A1–A24; the genes from the same subclade present similarity on gene structures and functional motifs). The family gene expansion might associated to the segmental and tandem duplications, and the duplicated genes were undergone purifying selection. The sequence homology of R2R3-MYB gene family was observed in A. carambola, Arabidopsis thaliana and Populus trichocarpa. RT-qPCR showed that except for AcMYB05, the genes AcMYB41, AcMYB65, AcMYB84, AcMYB87, AcMYB92, AcMYB97 and AcMYB100 were found with higher expression in T3 and T6 and rare transcripts in T1, fruit and leaves. Collectively, the transcriptional profiles suggested the tissue-specific expression of seven AcMYBs, which possibly participate in the lignin biosynthesis pathway of A. carambola, therefore providing reference for further analyzing the MYB family members that interplay with the lignin biosynthesis.
Key words:  Averrhoa carambola  lignin  MYB gene family  Genome-wide identification  expression pattern analysis

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