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一个南方水稻黑条矮缩病抗病基因发现及其初定位
李燕芳1, 冯 芳2, 肖汉祥1, 袁龙宇1, 周柏权3, 邱树青2, 张扬1
0
(1.广东省农业科学院植物保护研究所;2.武汉双绿源创芯科技研究院有限公司;3.珠海市现代农业发展中心)
摘要:
水稻南方黑条矮缩病是水稻主要病毒病害之一,挖掘抗源与抗性基因对水稻南方黑条矮缩病的抗病育种具有重要意义。本研究应用田间人工接毒和改良室内抗性鉴定技术,结合Reverse Transcription-Polymerase Chain Reaction(RT-PCR)检测,发现了一个育种群体对南方水稻黑条矮缩病出现抗感分离,暗示存在1个隐性抗病毒基因。通过对这个群体的单株进行抗病性鉴定,获得抗病和感病单株构建极端池,结合水稻全基因组育种基因芯片技术快速高效的进行基因分型,准确地完成了一个抗南方水稻黑条矮缩病基因的初定位,为南方黑条矮缩病的抗性育种提供理论支持。
关键词:  南方水稻黑条矮缩病  基因芯片  bulk segregant analysis (BSA)定位  抗性鉴定
DOI:10.13430/j.cnki.jpgr.20210423001
投稿时间:2021-04-23修订日期:2021-07-15
基金项目:特优高产抗逆广东丝苗米水稻新品种选育(2018B020202004);广东省现代农业产业技术体系水稻创新团队(2020KJ105)
Genetic Identification and Preliminary Mapping of A Resistance Gene Against Southern Rice Black Streaked Dwarf Virus
LI Yan-fang1, FENG Fang2, XIAO Han-xiang1, YUAN Long-yu1, ZHOU Bo-quan3, QIU Shu-qing2, ZHANG Yang1
(1.Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou;2.Wuhan Greenfafa Institute of Novel Genechip R&D Co Ltd;3.Zhuhai Modern Agricultural Development Center)
Abstract:
Southern rice black-streaked dwarf virus disease (SRBSDVD) is one of the most serious viral diseases. Exploration of resistant germplasm resource and identification of resistance-conferring gene become of interest in rice breeding. Here, by testing for SRBSDVD resistance in a breeding population by artificial inoculation under both field and greenhouse conditions followed by RT-PCR detection, individual plants showing a segregation on resistance and susceptibility were observed implying the presence of a new recessive virus resistance locus. We assembled the resistance and susceptibility bulks by pooling the resistant and susceptible plants, respectively. By genotyping using the whole genome breeding gene chip (GSR 40K) technology, this resistance gene against southern rice black-streaked dwarf virus disease was delimited in a lower resolution. Collectively, this study provided a resistance resource/gene valuable in resistance breeding against southern black streaked dwarf disease.
Key words:  SRBSDVD  gene chip  bulk segregant analysis(BSA)  resistance identification

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