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精细定位甜瓜白粉病抗性基因Pm-M
刘慧青1,2,谢丽琼1,2,王贤磊1,2,李 群1,2,宁雪飞1,2
0
(1新疆大学生命科学与技术学院,乌鲁木齐 830046;2新疆生物资源基因工程重点实验室,乌鲁木齐 830046)
摘要:
以抗白粉病甜瓜品种MR1与感白粉病新疆地方种新密1号为亲本,构建BC1和F2群体,研究白粉病菌P. xanthii race 1B的抗性遗传规律。以BC1群体和F2群体为试材,利用BSA(Bulked Segregation Analysis)结合分子标记技术发掘多态性信息,并开发分子标记进行抗性基因的遗传定位。抗病基因Pm-M位于LG12染色体上Indel2276和SSR12212两个标记之间,物理距离为75.5Kb (Chr 12:22765111 – 22840651),在该区段含有16个基因,其中12个候选基因可能与白粉病抗性相关。本研究的结果为抗病基因Pm-M的图位克隆及抗病分子育种奠定了基础。
关键词:  甜瓜  白粉病  精细定位
DOI:10.13430/j.cnki.jpgr. 20210506001
投稿时间:2021-05-06修订日期:2021-05-30
基金项目:新疆维吾尔自治区天山青年计划(2019Q065);新疆维吾尔自治区自然科学基金项目(2019D01C067);新疆大学博士科研启动基金项目(BS180228)
Fine Mapping of A Powdery Mildew Resistance Gene Pm-M in Melon
LIU Hui-qing1,2,XIE Li-qing1,2,WANG Xian-lie1,2,LI Qun1,2,NING Xue-fei1,2
(1College of Life Science and Technology,Xinjiang University, Urumqi 830046; 2Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046)
Abstract:
This study analyzed the genetic resistance gene against P. xanthii race 1B in BC1 and F2 populations which were derived from a cross of resistant parent MR1 and susceptible parent Xinmi1 in melon. By taking use of the bulked segregation analysis (BSA) using molecular markers, the polymorphisms between both pools (R vs S) were revealed followed by preliminary mapping and marker saturation in fine mapping. The segregation analysis revealed a single dominant resistance gene Pm-M against powdery mildew on chromosome LG12, flanked by SSR12212 and InDel 2276. The physical interval containing Pm-M was delimited to 75.5-kb (Chr 12:22765111-22840651), where 16 genes were annotated. Among them, 12 genes were considered to be candidates underlying the powdery mildew resistance. Collectively, this study laid a foundation for map-based cloning of the disease resistance gene Pm-M and its use in breeding for disease resistance melon varieties.
Key words:  melon  powdery mildew  fine mapping

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