引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

过刊浏览    高级检索

本文已被:浏览 278次   下载 0  
水稻愈伤组织超低温保存体系的建立
李天菲, 林田, 滕小英, 周丽, 韩静, 刘鸿艳, 罗利军
0
(上海市农业生物基因中心)
摘要:
水稻种质资源的长期有效保存对于世界粮食安全至关重要,而超低温保存是实现这一目标的最佳方式。本研究以水稻的胚性愈伤组织为材料,利用冻存后恢复培养阶段的新生愈伤率和新生愈伤分化率来评价不同品种不同粒级大小的胚性愈伤组织、不同浓度蔗糖预培养,以及高糖预培养和高糖预培-玻璃化处理2种预处理方式对水稻愈伤组织超低温保存效率的影响,并选用自主开发的15对水稻SSR标记引物,对超低温保存前后的愈伤组织分化的幼苗进行遗传多样性鉴定。研究结果表明,粳稻‘日本晴’和籼稻‘旱恢3号’1.6-3.0 mm和>3.0 mm粒径的愈伤组织超低温保存后的新生愈伤率极显著高于<1.6 mm的愈伤组织;‘旱恢3号’愈伤组织经100 g/L和170 g/L蔗糖预培养后的新生愈伤率极显著高于240 g/L蔗糖预培养。粳稻‘WDR48’、粳稻‘日本晴’和籼稻‘旱恢3号’仅采用高糖预培方法的超低温新生愈伤率都极显著高于玻璃化处理的新生愈伤率。简单序列长度多态性(SSLP)鉴定表明超低温保存前后的愈伤组织生成的幼苗遗传性稳定。本研究表明利用愈伤组织高糖预培养-直接超低温冻存是一种易操作、高通量、稳定可靠的中长期保存水稻种质资源的方法。
关键词:  水稻  愈伤组织组织  超低温保存
DOI:10.13430/j.cnki.jpgr. 20210510002
投稿时间:2021-05-10修订日期:2021-05-10
基金项目:上海市研发平台专项:上海市农作物种质资源共享服务平台(18DZ2293700)
Establishment of Rice Embryonic Callus Cryopreservation System
LI Tian-fei, LIN Tian, TENG Xiao-ying, ZHOU Li, HAN Jing, LIU Hong-yan, LUO Li-jun
(Shanghai Agrobiological Gene Center)
Abstract:
Effective long-term conservation of rice germplasm resources is crucial to world food security, and cryopreservation is the best way to achieve this goal. In this study, a method with rice embryogenic callus high sugar preculture and direct liquid nitrogen cryopreservation was established, and the callus regeneration rate and the differentiation rate of regenerated calli were used to evaluate the cryopreservation efficiency directly. The results showed that the callus regeneration rates of japonica rice ‘Nipponbare’ and indica rice ‘Hanhui 3’ with sizes of 1.6-3.0 mm and >3.0mm was significantly higher than those of <1.6mm after cryopreservation. The callus regeneration rates of ‘Hanhui 3’ after preculture with 100 g/L sucrose and 170 g/L sucrose were significantly higher than those after preculture with 240 g/L sucrose. The callus regeneration rates of japonica rice ‘WDR48’, japonica rice ‘Nihonbare’ and indica rice ‘Hanhui 3’ treated only with high sugar preculture were significantly higher than those treated with vitrification. Simple Sequence Length Polymorphism (SSLP) identification indicated that seedlings regenerated from calli either with or without cryopreservation were genetically stable. The study indicated that callus preculture with high glucose and direct cryopreservation is an easy, high-throughput, stable and reliable method for long-term preservation of rice germplasm resources.
Key words:  rice  callus  cryopreservation

用微信扫一扫

用微信扫一扫