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花生花斑种皮花青素生物合成相关miRNA 及其靶基因鉴定与分析
胡梦蝶1, 李佳伟1, 王 梅1, 刘 彬1, 马钰聪1, 赵雨露1, 杨鑫雷1, 崔顺立1, 侯名语1, 蒋晓霞2, 穆国俊1
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(1.华北作物改良与调控国家重点实验室/河北省作物种质资源实验室/河北农业大学;2.河北易园生态农业科技有限公司)
摘要:
花生在世界粮油食品中占有重要地位,其种皮颜色有白色、红色、紫色、粉红色及花斑等多种颜色,花斑种皮花生是其独特的成员之一,具有便于区分的优良性状。本研究以花斑种皮花生VG-02为研究材料,结果表明与花斑种皮颜色合成相关差异表达的miRNA富集的代谢途径有苯丙烷生物合成、类黄酮生物合成、异黄酮的生物合成,昼夜节律-植物。miRNA测序结果中共筛选出86个差异表达miRNA,其中20个差异表达的miRNA与花生花斑种皮颜色合成相关。与花生花斑种皮花青素合成相关的20个差异表达miRNA包括miR_8、miR_50、miR_51和miR_239-x ,这4个是共同靶向花青素、类花青素和IFS靶基因的miRNA;调控CHS靶基因的miR_398-x,调控4CL靶基因的miR_482,调控F3’H靶基因的miR_266和miR_182以及调控花青素3-O-葡萄糖苷靶基因的miR_5,这是5个靶向花青素生物合成中结构基因的miRNA;miR858-y靶向调控MYB2和MYB3,这是1个靶向花青素生物合成调节基因的miRNA;miR_10、miR_15、miR_61、miR_72、miR_102、miR_116、miR_123、miR_193、miR_256和miR_862-z这10个是靶向CYP450靶基因的miRNA。miRNA测序与转录组联合分析KEGG代谢通路表明类黄酮生物合成是花生种皮花斑形成最直接的代谢途径。本研究对于全面揭示花斑花生种皮花青素合成的分子机制有重要意义。
关键词:  花生  花斑种皮  花青素生物合成  miRNA  靶基因
DOI:10.13430/j.cnki.jpgr.20210519001
投稿时间:2021-05-19修订日期:2021-07-06
基金项目:河北省高等学校科学技术研究项目(ZD2019051);河北省重点研发计划项目现代种业科技专项(19226363D);易县易园农业科技园区建设(2111N004)
Identification and analysis of miRNA and its target genes related to anthocyanin biosynthesis in variegated testa peanut
HU Meng-die1, LI Jia-wei1, WANG Mei1, LIU Bin1, MA Yu-cong1, ZHAO Yu-lu1, YANG Xin-lei1, CUI Shun-li1, HOU Ming-yu1, JIANG Xiao-xia2, MU Guo-jun1
(1.National Key Laboratory of North China Crop Improvement and Regulation / Hebei Crop Germplasm Resources Laboratory / Hebei Agricultural University;2.Hebei Yiyuan Ecological Agriculture Science and Technology Co Ltd)
Abstract:
Peanuts are an important product as part of the world''s cereals, oils and foods. The testa colors of peanuts are white, red, purple, pink, and variegated. Variegated peanut testa are unique and can be distinguished from other types. In this study, the variegated testa peanut VG-02 was adopted as the research material. The study showed that the differentially expressed miRNA-enriched metabolic pathways related to the color synthesis included phenylpropane biosynthesis, flavonoid biosynthesis, isoflavone biosynthesis and rhythm-plant. MiRNA sequencing results showed that 86 miRNAs were differentially expressed of which 20 miRNAs were related to the color synthesis in variegated testa peanut. Twenty differentially expressed miRNAs related to anthocyanin synthesis in variegated testa peanut including miR_8, miR_50, miR_51 and miR_239-x, that jointly targeted anthocyanins, anthocyanidins and IFS target gene. Five miRNAs were found to targete structural genes in the anthocyanin biosynthesis: miR_398-x which regulates CHS target genes, miR_482 which regulates 4CL target genes, miR_266 and miR_182 which regulate F3''H target genes and miR_5 which regulates the anthocyanin 3-O-glucoside target gene. MiR858-y is a miRNA targeting anthocyanin biosynthesis regulation gene, which targets and regulats MYB2 and MYB3. MiR_10, miR_15, miR_61, miR_72, miR_102, miR_116, miR_123, miR_193, miR_256 and miR_862-z target CYP450 target genes.The joined analysis of KEGG metabolic pathways by miRNA sequencing and transcriptome analysis indicated that flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. This study further our understanding of the molecular mechanisms of anthocyanin synthesis in variegated testa peanut.
Key words:  peanut  variegated testa  anthocyanin biosynthesis  miRNA  target gene

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