wheat varieties (lines) were identified for resistance to different races of Puccinia striiformis f.sp tritici, a causal agent of wheat stripe rust, in Gansu during 1994-2002. The results showed that the resistant level of winter wheat was higher than that of spring wheat and the resistance of Gansu's varieties was better than that of varieties collected from other provinces. The results in the fields indicated that the varieties released in the main wheat production areas were susceptible to stripe rust and only a few Gansu's varieties, such as Longjian 127, were resistant. Some of resistant sources used in breeding at present were resistant and some became susceptible. By artificial inoculation with different races, field monitoring for resistance to stripe rust and powdery mildew and survey of agronomic characteristics, 20 accessions were identified as good sources for breeding in the future.