Through investigation of the 29 varieties of L.esculentum in the locae germplasm repository, the results indicated that there were at least 10 pairs of morphological genic variations,which could provide the rich potential of different genetic recombination. It reflected to certain extent that the germplasm repository had the potential to preserve genetic diversity. The preliminary results through examining the fruit quality of 7 F 1 using 6 parents showed that some qualities of the F 1 would surpass those of the marketing varieties. By using 18 RAPD primers to amplify DNA of 6 parents and their 8 F 1, the results showed that 12 different primers varied in the relative ability for showing the polymorphism of the varieties. The primers that can both amplify and efficiently show the polymorphism were A11, K05, C15 and A17, in which K05 and A11 could produce most amplified bands. Furthermore, the primers A11 and C19 could be used to examine the purity of 2013B, and B11 to 2018A.