In this study, 27 newly collected wild soybean materials and 38 conserved germplasm collected from the same places were used as materials. Sixty SSR primers were used to analyze the genetic diversity of these materials. The objective was to illustrate the necessity of collecting wild soybean materials from the place where soybean had been collected before. 62 new alleles were found only to exist in the newly collected materials, and in 22 loci, the genetic diversity index of newly-collected materials was higher than the conserved germplasm. The average similarity coefficient of newly-collected materials with other ones was 0.1918. The results indicated that it was valuable to recollect wild soybeans.