Using 89 2343, a durum wheat addition line with Ms2 and blue seed, crossed and backcrossed with common wheat line 7739 3 to produce fertile plants from blue seeds (MFP BS). To add the blue seed marker to Rht10 and Ms2, short male sterile plants were fertilized by pollen of selected MFP BS. Thirteen dwarf male sterile wheats with blue seed marker were bred based on the different genotypes. The segregation ratios of male sterility and seed color as well as chromosome figure of different plants indicated that the blue grain genes, Ms2 and Rht10 were located on the same additional chromosome. Cytological analysis showed that the blue marker male sterile lines in common wheat was monosomic with an additional chromosome. The inheritance ratio for blue seed male sterile plants and white seed male fertile plants in progeny was 22 1% and 77 7%, respectively. Moreover, the potential application for blue marker sterile lines in population improvement and hybrid production is discussed.