S511.035.3
国家科技攻关项目,离子束应用技术研究项目
利用早籼品系HZP145、 K17A、金23A为亲本,与离子束介导玉米全DNA导入的转基因水稻株系中的单株配制杂交组合.分析其杂种F1群体的主茎穗结实率、株高、单株有效穗的变异特点及抽穗整齐度状况,以此推断转基因水稻材料分离的形成原因.结果表明,转基因水稻材料分离的形成与其核基因组的遗传不稳定性有关,而与玉米基因片断导入受体细胞质以及环境因素的影响无关.文章还对转基因水稻材料在植物遗传育种中的意义及价值进行了讨论.
Based on the analysis of the variation of effective panicles, plant height, seed set of main culms panicle, heading of F 1 populations from crosses betweena transgenic rice line and HZP145, K17A, JIN23 A, respectives. It was found that the genetic instability of the transgenic rice line was introduced to the instability of nuclear genome of the transgenic rice line, and its expression could not be affected by cytoplasm of the transgenic rice line itself. The potential value of the transgenic rice line was also discussed.
童继平,刘学军,吴跃进,等.离子束介导玉米全DNA导入转基因水稻遗传稳定性分析[J].植物遗传资源学报,2004,(2):123-127.
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