S681.2 S682.33
以非洲紫罗兰叶片为材料进行胚状体诱导及快繁技术研究.结果表明:.在MS NAA0.1mg/L BA0.1 mg/L 2,4-D1.0 mg/L的培养基上培养15d利于诱导胚性细胞分化,起始黑暗培养5~10d可提高胚性细胞分化率;在MS BA0.05~1mg/L的培养基上可诱导胚状体大量发生;在MS NAA0.1mg/L十BA0.1 mg/L的培养基上能够获得茎芽快速增殖;在1/2MS NAA0.01mg/L的培养基上可以生根.
Somatic embryoid of Saintpaulia ionantha was obtained from the leaf explants thought rapid propagation.Embryonic cells were preliminary induced on MS+BA0.1mg/L+NAA0.1mg/L+2,4-D1.0mg/L for 15 days,and their regeneration could be increased by 5-10 d initial dark culture.Embryoid could be easily induced on MS+BA0.05-1mg/L.Rapid propagation medium was MS+NAA0.1mg/L +BA0.1 mg/L.Roots could be easily induced on 1/2 MS+NAA0.01mg/L.
师嘉临,师桂英,任治斌,等.非洲紫罗兰叶片体细胞胚培养及快繁技术研究[J].植物遗传资源学报,2005,(4):453-456.
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