S513.035.1
中国农业科学院科研项目
采用包括两套近等基因系在内的11份含有不同抗大斑病基因的玉米材料,对已报道的与Ht2、Ht3基因连锁的分子标记进行应用性检测。实验选用umc1202、bnlg1152、umc1149、SD-06633和bnlg1666等5对引物进行PCR扩增检测,发现被检测引物均缺乏对相应标记基因的特异性扩增;对与Ht2基因连锁的SCAR引物SD-06633的扩增片段进行了序列分析,发现其在Ht2和HtN基因背景下的扩增片段长度均为631bp,且序列相似性高达98.73%,扩增产物特征一致,无法证明该标记的特异性。检测结果表明,上述已发表的与抗大斑病基因Ht2和Ht3紧密连锁的5个分子标记缺乏特异性,不适用于玉米材料的Ht2和Ht3基因型鉴定。
11 corn inbred lines with different Ht gene background were used for detecting the practicability of 5 molecular markers linked to Ht2 and Ht3 genes.Primers,umc1202,bnlg1152,umc1149,SD-06633 and bnlg1666 were detected for their specific to Ht gene.No specific amplification was found in all of the primers.Analysis of the fragments amplified by primer SD-06633,linked to Ht2 gene,showed that the sequence identity was 98.73% between lines with Ht2 and HtN gene.It means that the primer,SD-06633,can not amplified specially for Ht2 gene.None of the 5 primers can be used to identify the present of Ht2 or Ht3 gene in corn.
程品冰,王晓鸣,高卫东.玉米抗大斑病基因Ht2、Ht3分子标记的应用检测[J].植物遗传资源学报,2007,(3):285-288.
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