S513.035.1
国家科技支撑计划;中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项资助项目
利用70对SSR引物,对30份玉米地方品种种质进行遗传完整性检测,每一份种质包含来自两个不同繁种年份的供试亚群体。取样方法为DNA混合取样。结果表明,30份种质的供试亚群体之间的遗传相似系数除红包谷(统一编号00230080)为0.77外,其他都大于0.80。聚类分析表明,除二黄(统一编号00210055)和红包谷两份种质的亚群体出现分支外,其他28份种质亚群体分支都按照同一种质群分别聚类。本文同时探讨了个别种质亚群体之间遗传分化的原因,以及SSR标记作为玉米种质遗传完整性检测方法的可行性。
The genetic integrity of 30 accessions of maize(Zea mays L.) stored in national genebank of China was investigated by 70 SSR primers.Each accession was represented by two sub-populations regenerated in different year.The genetic similarity between the two sub-populations of the same accession is all above 0.80,except for 0.77 in Hongbaogu(accession number 00230080).Cluster analysis based on the similarity coefficient showed that Er-Huang(accession number 00210055) and HongBaogu diverged from each other,falling into two branches,and that each one cluster was created by two sub-populations of the same accessions,which were multiplied in different years.The reasons for the cluster divergence and feasibility of SSR for genetic integrity analysis in maize were discussed.
马延飞,卢新雄,陈晓玲,等.基于SSR标记的30份玉米种质遗传完整性分析[J].植物遗传资源学报,2007,(4):387-391.
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