Two new materials(N/BBCC and J/BBCC) with the similar nucleus genome from Ethiopia mustard(Brassica carinata) and different cytoplasm from Brassica napus and Brassica juncea respectively were obtained by backcrossing the B.napus and B.juncea seven generations with Ethiopia mustards as the transmigration parent.The new materials(N/BBCC and J/BBCC) and their transmigration parent(C/BBCC) put up the similar phenotypes in development stage,morphological characters,POD enzyme activity,and so on.But differences in male fertility,proline content and relative conductance rate were existed among them,in which the development of androecium in N/BBCC was abnormal,showing serious male sterility,and the proline content and relative conductance rate in N/BBCC and J/BBCC were much lower than their transmigration parent,C/BBCC.