The genetic diversity of 22 wheat cultivars from Henan province of China was analyzed using SRAP-PCR method. The results showed that 10 pairs of SRAP primer combinations produced 169 bands, of which 70 bands were polymorphic with 41.42% of polymorphism, average 7 polymorphic bands per primer pair. All the polymorphic alleles of 22 wheat cultivars were recorded, and a cluster analysis among them was also made using UPGMA. The genetic relationships among these wheat cultivars can be characterized by SRAP-PCR, a good way for wheat genetic diversity study.