采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)对236份新疆小麦地方品种的高分子量麦谷蛋白亚基(HMW-GS)的组成进行了分析。结果表明:Glu-1位点共有19种等位基因,其中Glu-A1位点3种,Glu-B1位点7种,Glu-D1位点9种;亚基null、7+8、2+12在各自的位点上出现频率最高,分别达到91.95%、85.17%、80.93%;亚基组成类型共有21种,主要为null/7+8/2+12,频率达70.34%;同时筛选出33份含有1、2*、13+16、14+15、5+10、1.5+10、17+18等优质亚基的材料,可作为优质基因源。利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)对其中的65份地方品种进行醇溶蛋白多样性分析。结果表明:电泳出现64条迁移率不同的谱带,构成65种组合,其中ω区出现的谱带最多,达17条,其次是β和γ区各16条,α区出现的谱带数最少,为15条。从每条谱带在65份材料中出现的频率看,总的变异范围为1.54%~93.85%;α、β、γ和ω四个分区多样性指数(H′)分别为0.498、0.386、0.523和0.348。这表明新疆麦区小麦地方品种贮藏蛋白位点存在丰富的遗传多样性。
In order to know the genetic diversity of wheat landraces in Xinjiang wheat production area and provide basic materials for improving wheat quality, subunits composition of high molecular weight gluten with a total of 236 wheat landraces were analyzed by means of SDS-PAGE. The result indicated that there were 19 alleles on Glu-1 loci, including 3 alleles on Glu-A1 loci, 7 alleles on Glu-B1 loci, 9 alleles on Glu-D1 loci; And null, 7+8,2+12 subunits occurred with highest frequency on each of loci, reaching 91.95%,85.17%,80.93% respectively. There are 21 types of subunit compositions and predominant was null/7+8/2+12 with a frequency of 70.34%. Materials conferred with elite subunits of 1, 2*,13+16, 14+15, 5+10,1.5+10, 17+18 were selected and could be a source of desirable genes. Meanwhile , Gliadin of 65 wheat landraces in Xinjiang wheat production area were analyzed by A-PAGE. It was shown that 64 different spectral gliadin bands were isolated from the materials; the bands including 17 bands in ω region, 16 in β and γ regions and 15 in α region, and their frequencies ranged within 1.54%~93.85%. Genetic diversity index(H′ ) of α, β, γ and ω regions were 0.498,0.386,0.523,0.348, respectively. High genetic diversity of storage proteins in wheat landraces of Xinjiang wheat production area was observed.
陈晓杰,吉万全,王亚娟.新疆冬春麦区小麦地方品种贮藏蛋白遗传多样性研究[J].植物遗传资源学报,2010,11(10):522-528.
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