In order to analysis the genetic basis of landrace soybean in Hebei province, 50 landrace soybean were used as materials, the phenotype of 12 agricultural traits were invested in 2 years and the genotype of 30 SSR loci were screened. The result showed that the PIC of agricultural traits was 0.83 and the CV was 20.15% in 2 years. 5.03 alleles were detected at each SSR locus on average, the average PIC of 30 SSR loci were 0.61, in addition, the average genetic distance was 0.869. 50 landrace were separated into 4 groups on Bayesian model. As a result, there was no significant difference among landrace soybean from different region of Hebei province.