Inheritance of oleic,linoleic acid content and O/L ratio in two crosses of high oleic acid Arachis hypogaea Lvar.hirsuta low O/L ratio var.vulgaris,were investigated by applying the major gene plus polygene model of quantitive traits analysis of F2 generation.The results showed that both oleic and linoleic acid content were controlled by one major gene with additivedominance effects.The estimated additive,dominant effects and heritability of the major gene for oleic acid were 8.6281,-2.0164 and 65.26% in cross I,and those in cross II were 10.6638,1.0652 and 71.39% respectively.For linoleic acid,corresponding values were 8.0327,1.2858 and 73.64% in cross I,and 9.0885,-1.0826 and 71.59% in cross II.The O/L ratio was controlled by two major genes with additivedominanceepistatic effects.The estimated additive effects of the two major gene were 0.6855,0.6814 in cross I,and 1.6842,0.8835 in cross II.The dominant effect of the two major gene were -0.6838,0.024 in cross I,and -1.6559,-0.5127 in cross IIThe interaction effect values of i,jab,jba and l were 0.6812,0.024,-0.6803 and -0.0244 in cross I respectively,while those were 0.8822,-0.5124,-0.8594,and 0.496 in cross II.Heritability values of the major genes was 82.57% in cross I and 88.64% in cross II.The results that,both oleic and linoleic acid content were controlled by one major gene while O/L ratios were controlled by two major genes indicated that two different major genes controll oleic and linoleic acid content respectively.