国家高技术研究发展计划(863计划)项目(2006AA100101);国家科技支撑计划项目(2006BAD01A01-5);农业部行业科研专项(nyhyzx07-001-006);江苏省农业科技自主创新基金(CX[08]603)
突变体是研究植物基因功能和培育优质高产作物新品种的重要材料。本研究以半矮秆广亲和粳稻亲本02428为父本,以其半矮秆突变体02428ha为母本进行杂交,从其 F6稳定群体中筛选到3个双矮品系08-4、08-12和08-19,2008年测量其株高分别为62.4±3.3cm、66.0±1.5cm和67.5±1.7cm。分别以3个双矮品系为亲本,与南京6号、南京11号及培矮64S杂交进行遗传分析,结果表明,3个双矮品系的矮生性状均受两对隐性半矮秆基因控制,其中1对基因与半矮秆基因sd1等位,另1对为与sd1不等位的隐性半矮秆基因sd-h(t)。本研究为半矮秆基因sd-h(t)的分子定位、基因克隆及其作用机理的探究奠定了基础。
Mutant is an important material for investigating gene function of plant and developing new crop variety with good quality and high yield.In the study,three double-dwarf lines 08-4,08-12 and 08-19,with dwarfism stably inherited,were selected from the F6 progeny of 02428ha/02428.Plant height of three lines were 62.4±3.3cm,66.0±1.5cm and 67.5±1.7cm.Genetic analysis showed that the dwarfisms were controlled by 2 recessive genes,one gene was allelic to sd1,while the other one was a novel recessive semidwarf gene sd-h(t),which was not allelic to sd1.These results provided a basis for mapping and molecular cloning and functional analysis of sd-h(t).
陈仁霄,张所兵,赵凌,等.来源于02428ha的水稻双矮品系的获得与鉴定[J].植物遗传资源学报,2010,11(1):65-71.
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