国家高技术研究发展计划(863计划)
本文利用HPLC法分析了50份遗传背景丰富的白菜型油菜、甘蓝型油菜、芥菜型油菜和芸芥种子油中维生素E的组成与含量,研究结果显示,油菜种子油中主要含α-生育酚和? -生育酚,且α-生育酚、? -生育酚和维生素E总量均存在明显的基因型差异,甘蓝型油菜种子油中维生素E含量总体水平最高,平均总量较高,为123.11 mg/100g油, 维生素E含量最高的Omega,总量为144.73 mg/100g油, α/ ? -生育酚比值最高可达0.77。α-生育酚、? -生育酚和维生素E总量与类胡萝卜素含量均呈现显著以上负相关,种子油中α-生育酚与含油量呈现显著正相关,α-生育酚、? -生育酚和维生素E总量与生育期均呈现显著或极显著正相关,α-生育酚和维生素E总量与株高均呈现显著正相关,维生素E总量与千粒重呈显著正相关,而α-生育酚、? -生育酚和维生素E总量与全株角果数和每角粒数相关不显著。
Seed oil tocopherol content in 50 divergent genotypes of oilseed rape of Brassica campestris L., Brassica napus L., Brassica juncea Coss.and Eruca stiva Mill. were analysed by HPLC method, the results indicated thatα-tocopherol andγ-tocopherol are the two dominant tocopherols in seed oil, and significant variation in both composition and content could be found among different genotypes. tocopherol content in seed oil of Brassica napus L. is the highest among the species, with a average content of 123.11 mg/100g oil, the genotype Omega has the highest total content of 144.73 mg/100g oil, the highest ratio of α-tocopherol andγ-tocopherol is 0.77. α-tocopherol ,γ-tocopherol and total tocopherol content showed significant negative correlation with carotenoid content, a significant positive correlation were found betweenα-tocopherol content and oil content. α-tocopherol ,γ-tocopherol and total tocopherol content presented significant positive correlation with growth peroid, α-tocopherol and total tocopherol content are significantly correlated with plant height, total tocopherol content demonstrated significant positive correlation with 1000 seeds weight. However, α-tocopherol ,γ-tocopherol and total tocopherol content showed no correlation with number of siliques and Seeds number per silique.
吕培军,薛蕾,伍晓明. HPLC分析油菜籽油中维生素E的组成与含量[J].植物遗传资源学报,2011,12(4):634-639.
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