Extraction of high-quality RNA is the base for seeds molecular biology research. Rapid isolation of high-quality total RNA from dry seeds is difficult by the extraction methods reported previously. In this new protocol, SDS and guanidinium thiocyanate methods were combined for RNA extraction and high-quality of total RNA was obtained in about 3.5h. Extraction at low pH, precipitation of polysaccharides with potassium acetate, usage of phenol blocker polyvinylpolypyrrolidone(PVPP) yielded high-quality RNA from rice, soybean, broad bean, kidney bean and peanut with a small number of samples of 0.01-0.1g. The extracted RNA was suitable for RNA reverse transcription and RT-PCR. This method can provide some references for isolating total RNA from dry seeds of other species with similar chemical composition.