农业部948专项(2004-Z30、2005-Z18) 上海市科委技术标准专项(05DZ05003)
DUS(特异性、一致性和稳定性)测试是进行新品种申请的必要步骤。本文以28个不同的西瓜品种为研究对象,分别采用21对SRAP引物标记和54个用于DUS测试的形态学标记对其进行遗传多样性分析,其中SRAP引物在不同品种间的多态信息含量(PIC)在52.5%~89.2%之间,平均值为72.0%,计算得到的各材料间相似系数在0.92至0.99之间,而形态学标记统计得到各材料间相关系数在0.50到0.85之间。采用UPGMA法对所有材料进行聚类分析,SRAP分子标记聚类划分成四类,形态学标记将其划分为五类。对两种标记所得的结果进行相关性分析得出两者的相关系数为0.218,表明形态学标记和SRAP标记在这些材料上表现的相关性不是很高,但在品种鉴定和区别上SRAP标记表现出一定的优势,可以作为DUS测试的一种有益补充。
The tests of distinctiveness, uniformity, and stability(DUS) are necessary steps for varieties identifing and new varietis applying. 21 SRAP markers and 54 morphological traits for DUS tests were used for diversity analysis of 28 watermelon(Citrullus lanatus) varieties. Average polymorphism information content(PIC) of the SRAP markers was 0.72, ranging from 0.525 to 0.892 and the correlation coefficients of varieties ranging from 0.92 to 0.99. The correlation coefficients range from 0.50 to 0.85 in morphological traits test. Clusters analysis of the band patterns using UPGMA analysis separated the varieties to four (SRAP markers) and five (morphological) groups, showing certainsimilarity with in them.The correlation coefficient of two markers is 0.218, showing of no significance correlation. But the SRAP markers show some advantages in varieties identification that could be a good supplement for DUS test .
刘丽娟,陈海荣. SRAP标记与形态学标记在西瓜DUS测试中的比较[J].植物遗传资源学报,2011,12(5):790-795.
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