公益性行业(农业)科研专项(3-27)
Special Fund for Agro-scientific Research in the Public Interest
采用形态学方法对采集于新疆野生的阿魏蘑标本进行分类鉴定,结果表明其形态学特征符合刺芹侧耳托里变种Pleurotus eryngii var. tuoliensis的特征范围,它与欧洲已报道的Pleurotus nebrodensis有明显的差异。此外本研究还采用分子生物学方法对从该野生样品分离的菌株CCMSSC 02514进行了rDNA ITS序列分析,结果表明它与我国栽培白灵菇菌种CCMSSC 00973、KH5和AFRL 6022完全相同,以此构建系统发育树,将我国栽培白灵菇种质与意大利的Pleurotus nebrodensis、Pleurotus eryngii var. ferulae以及来自荷兰的Pleurotus eryngii等截然分为两组。因此,形态学和rDNA ITS序列分析结果支持我国栽培的白灵菇与欧洲的Pleurotus nebrodensis为不同种,我国的白灵菇是刺芹侧耳独立进化的一个分支,其名称应该为刺芹侧耳托里变种Pleurotus eryngii var. tuoliensis。
A wild A-Wei-Mo specimen collected from Xinjiang was identificated with morphological methods. The results showed that it was consistent with the morphological characteristics of Pleurotus eryngii var. tuoliensis, but different from those of Pleurotus nebrodensis reported in Europe. In addition, the strain CCMSSC 02514 isolated from the wild mushroom specimen was analyzed with molecular menthods. Based on rDNA ITS sequences from the phylogenetic tree, the relationship among Bai-Ling-Gu in China, Pleurotus nebrodensis and Pleurotus eryngii var. ferulae in Italy, and Pleurotus eryngii are analyzed. The rDNA ITS sequences of CCMSSC 02514 and Pleurotus eryngii var. tuoliensis AFRL 6022 are identical. Combined with morphology and rDNA ITS sequence analysis, we considered that Bai-Ling-Gu in China is different from the European Pleurotus nebrodensis, and the former is a branch of Pleurotus eryngii evolved independently in China. The scientific name for the Chinese Bai-Ling-Gu in China should be Pleurotus eryngii var. tuoliensis.
张金霞,黄晨阳,陈强,等.中国栽培白灵菇学名的订正[J].植物遗传资源学报,2011,12(5):825-827.
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