用基因工程技术培育百号抗病品种研究(1004TCYH007)
为了揭示罂粟(Papaver somniferum L.)种质的遗传多样性,利用简单重复序列区间 (inter-simple sequence repeat,下称ISSR) 分子标记进行遗传多样性研究,从100条随机引物中筛选出9条多态性引物对所有样品扩增,共检测到109个等位变异位点,引物等位位点数在9-16之间,平均每条引物有12个等位变异,其中,引物807的等位位点最多,为16个。引物等位位点多态性信息量PIC值为0.77~0.92。其中引物807的PIC值最大(0.92),引物847的PIC值最小(0.77),遗传相似系数在-0.45~0.98之间,且明显聚为2个类群;亲缘关系和地理分布呈一定的相关性,但没有形成明显的地理变异模式;本研究将为罂粟种质资源科学有效利用提供理论依据。
In order to reveal the genetic diversity of poppy in different ecological zones. Based on the molecular markers of 100 inter-simple sequence repeat ( ISSR), poppy and relatives from 12 different areas were amplified and analyzed with optimizing conditions for the primers. The results showed that a total of 109 allelic variations were detected, between 7-16 loci for each primer. Among them, primer 807 had the most alleles of 16. The value of allelic polymorphism information content (PIC) ranged from 0.77-0.91, which the Maximum is 807 (0.92) and the minimum is 847 (0.77). The results showed that the genetic similarity among them ranged from -0.45 to 0.98, and these 12 germplasms were classified into 2 groups. The poppy germplasms sources was less correlated with geographic latitude . There was no evident geographic patterns of the poppy germplasm sources happened. The research will provide basis theoretical for scientific and effective utilization poppy germplasms.
魏玉杰,张金文,何庆祥,等.不同生态区罂粟种质的遗传多样性ISSR分析[J].植物遗传资源学报,2012,13(2):239-243.
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