The genome contents of 14 major cruciferous vegetables were measured. The genome contents of green-kohlrabi (Brassica oleracea L. var. caulorapa D.C.) , savoy (Brassica Chinensis var. rosularis Tsen et Lee.) , leaf mustard (Brassica juncea var. multiceps Tsen et Lee.) and Chinese kale (Brassica alboglabra Bailey) were firstly reported in the current study. Comparing the relevant data and numerical of foreign literature provided, the genome contents of the green radish (Raphanus sativus Bailey) , cabbage (Brassica oleracea L. var. capitata) , broccoli (Brassica. oleracea L. var. italica) and root mustard (Brassica juncea Coss) were basically consistent with the reported values, the genome contents of the heading Chinese cabbage (Brassica campestris L. var. cephalata Tsen et Lee) and cauliflower (Brassica oleracea L .var .botrytis L.) were some different from the reported data. The reasons which cause the variation in genome content of the same species were possibly the difference of breed, the diverse growing environment or the inconsistent reference standard.