In order to acquire the difference among Vitis species and individuals, the photosynthetic parameters of twenty-five lines in seven species were measured using Li-6400 portable photosynthesis system. The results showed that photosynthesis characteristics was significantly different among wild Vitis species, Pn of Vitis bellula was the highest, the second was V. davidii ,and V. adenoclada was the lowest .The diversity of photosynthesis indexes among different wild Vitis individuals was abundant. The difference among individuals of V. flexuosa was the biggest. If the climates of origin were similar, the photosynthesis characteristics were alike. If the climates of origin were significantly different, the photosynthesis characteristics were significantly different. The regulation mechanism of Pn was diverse, and the non-stomatal regulation factors were universal in wild Vitis.