The methods of eliminating walls by enzymolysis and low osmosis were used to analyze the chromosome karyotype of three Chinese jujube cultivars.The results showed that the chromosome numbers of Ziziphus jujuba Mill. ‘Dalilongzao’, Ziziphus jujuba Mill. ‘Junzao’ and Ziziphus jujuba Mill. ‘Dongzao’ were 2n=24, the karyotype formula was 2n=2x=24=20m(4SAT)+4sm,2n=2x=24=20m(2SAT)+4sm and 2n=2x=24=16m(4SAT)+8sm respectively, and the karyotypes of all were 2B type. Comparatively, the karyotype of Z. jujuba Mill. ‘Junzao’ was original, followed by Z .jujuba Mill. ‘Dalilongzao’ and the karyotype of Z. jujuba Mill. ‘Dongzao’ appeared most evolutional.