A new male sterile line Ji 130A was developed after backcrossing Hui 18A with an aphid-resistance parental line 130B for 8 generations. It was discovered that temperature affects sterility of Ji 130A. In order to understand the effect of temperature to sterility of Ji 130A , confirm critical temperature for the hybrid production, the Ji 130 was sown on different dates in different growing sites and various temperature conditions at key growing stages were applied. The results showed that Ji 130A was completely sterile when the mean temperature was below 23℃ during period from flag leaf to heading but fertile pollen appeared when temperature was above 23℃. Its selfing rate could reach 30% with variation among individual plant when temperature was over 26℃. The development of its anther and pollen under various temperature conditions was characterized by using paraffin sectioning and microphotography. The results showed that under low temperature conditions the tapetal cells appeared obvious deformity including vacuolation, radial expansion and delayed degradation. The sporegenous cell or pollen mother cell of early abortive anther disintegrated earlier than normal and no mononuclear pollen developed. When the mean temperature was over 26℃ during period from flag leaf to heading mononuclear pollen developed with deformity but some about 40% were normal and could be released from anther. Low temperature causes tapetal cells abnormal, which may be the main reason causing pollen abortion. Ji 130A was identified and developed, it diversifies the cytoplasm and nuclear genetic base of sorghum hybrids and is beneficial to the commercial hybrid breeding programs. Ji 130A can be as a new germ plasm to research the pollen abortion mechanism, the research on the characteristics of fertility alteration lays a foundation to select the region and sowing time for hybrid production.