浙台科2011(125)号
对浙江省东南部台州、温州、宁波及绍兴4个地区25份杨梅地方品种材料的气孔特性进行研究,测定了气孔密度、气孔面积、气孔长、短轴和气孔器长、短轴。结果表明:所有材料气孔均属于平行型;气孔密度在500-1200个/ mm2之间,主栽品种东魁、荸荠和丁岙梅气孔密度偏小;气孔面积从40-80μm2不等,以50μm2较为集中;气孔器长、短轴以未知1号最大,温州土梅最小;气孔长、短轴的变化规律为长轴>短轴。利用重心距离法对气孔密度、气孔面积、气孔大小和气孔器大小进行聚类分析,从距离0.5处进行分类,可分为5类。通过聚类分析表明:气孔的相似度在某种程度上可以作为区别品种的依据,但要精确的研究它们之间的遗传关系,还需与分子生物学、形态学等综合手段相结合予以鉴定。 关键词:杨梅;气孔特征;显微观察;聚类分析
The stomatal density, stomatal area, size of stoma and stomatal apparatus of 25 Chinese bayberry(Myrica rubra Sieb.et Zucc)leaves in southeast of Zhejiang province Taizhou.,Wenzhou.,Ningbo and Shaoxing The stomatal density, stomatal area, size of stoma and stomatal apparatus of 25 Chinese bayberry(Myrica rubra Sieb.et Zucc)leaves in southeast of Zhejiang province Taizhou.,Wenzhou.,Ningbo and Shaoxing were examined by optical microscope. The results showed that stomata of all varieties were of parallel type; stomatal density ranged from 500-1200 No./ mm2, and major cultivars, such as Dongkui, Biqi and Ding’aomei presented a low density level; size of stomatal area concentrated in 50μm2 at a range of 40-80μm2; Weizhi No.1 had the largest major and minor axis of stomatal apparatus size, while Wenzhoutumei had the smallest; the major axis were bigger than the minor axis of all stoma size. Cluster analysis of stomatal density, stomatal area, size of stoma and stomatal apparatus by center of gravity method, classification from a distance of 0.5, it can be divided into five categories A cluster analysis showed that it was, to a certain extent, practicable to distinguish the varieties by using stomatal similarity. However, molecular biology and morphology methods were also needed for precise study of the genetic relationships. Keywords: Chinese bayberry; sStomatal characters; mMicro observation; cCluster analysis
陈方永,王引,倪海枝,等.浙东南杨梅叶片气孔观察与相似性研究[J].植物遗传资源学报,2012,13(4):626-631.
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