对海岛棉产量和早熟性相关性状进行QTL初步定位,为利用分子标记辅助选择提高海岛棉育种效率提供依据。以海岛棉品种新海3号和吉扎82为亲本构建的190个F2:3家系为作图群体构建海岛棉的遗传连锁图谱,从5000多对SSR引物中筛选出107对在双亲间多态性明显的引物,利用JoinMap3.0分析软件构建了一个包含22个连锁群,74个标记,标记间平均距离12.06cM,全长893cM,覆盖海岛棉基因组20.12%的分子标记遗传连锁图谱。采用复合区间作图法检测到21个与海岛棉产量性状和早熟性状有关的QTL,其中早熟性状检测到12个QTL,分别位于1、3、5、6、11、17、22共7个连锁群上,产量性状检测到9个QTL,分别位于1、4、5、6、16、22共6个连锁群上。研究结果为棉花产量性状和早熟性状的分子设计育种提供了有用的信息。
The yield and earliness-related traits of the sea-island cotton were identified to provide DNA molecular markers for using in cotton improvement in the future. A genetic linkage map of sea-island cotton was constructed by using 190 F2: 3 family lines derived from two sea-island cotton cultivers (Xinhai 3 and Jiza 82) with 107 polymorphic primer pairs obtained by screening among 5000 SSR primer pairs. The linkage map covered 20.12% of the whole cotton genome which including 22 linkage groups, 74 markers, a whole length of 893 cM with an average interval of 12.06 cM between two markers by Joinmap3.0 software. Based on composite interval mapping. 21 QTLs related with yield and earliness traits of the sea-island cotton were detected. 12 QTLs associated with earliness traits were respectively located on linkage groups LG1 LG3 LG5 LG6、LG11、LG17, LG22 , and 9 QTLs associated with yield traits were respectively located on linkage groups LG4、LG5、LG6、LG16、LG22. This research could provide valuable information for the molecular design breeding of yield and earliness in sea-island cotton.
张薇.海岛棉产量和早熟性相关性状的QTLs定位[J].植物遗传资源学报,2012,13(4):614-621.
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