- 摘要 | |
- 访问统计 | | | | |
- 文章评论
以11个苹果品种为试验材料，根据保守氨基酸序列“FTQQYQ”和“anti－1/WⅠPNV”设计苹果自交不亲和基因引物，利用酶切分析和目的片段DNA测序方法鉴定了9个新的S等位基因，将该9个S-等位基因分别标记为：S34-、S35-、S36-、S37-、S38-、S39-、S40-、S41-、S42-等位基因，在GeneBank中的接收号依次为：EU310474、EU391605、EU391606、EU391607、EU391609、EU391610、EU391611、EU391612、EU391613。11个苹果品种的自交不亲和基因型分别为：桑萨(S40S40)、芳明(S1S9)，烟嘎1(S38S27)、红金嘎拉(S39S27)、烟嘎2(S38S27)、青9号(S41S42)、阿斯(S36S36)、皇家嘎拉(S37S27)、静香(S1S9S34)、高岭(S38S9)、红奥(S35S35)。
The self-incompatibility allele of 11 apple cultivars were tested in this article．Self-incompatibility apple primers were designed according to the conserved sequence of amino acid“FTQQYQ”and“anti－1/WⅠPNV”． In this article, 9 new self-incompatibility genes named S34-, S35-, S36-, S37-, S38-, S39-, S40-, S41-, S42-Allele respectively were isolated with enzyme digestion and DNA sequencing of aim fragment method, they were deposited in GeneBank under the accession numbers of EU310474, EU391605, EU391606, EU391607, EU391609, EU391610, EU391611, EU391612 and EU391613. The self-incompatibility Alleles of 11 apple cultivars were: Sansa(S40S40), Fangming(S1S9), Yanga1(S38S27),Hongjingala(S39S27),Yanga2(S38S27),Qing9(S41S42),Asi(S36S36),Huangjiagala(S37S27), Jingxiang(S1S9S34),Gaoling(S38S9), Hongao(S35S35).
引用本文
张雪梅.11个苹果新品种自交不亲和基因的分离与鉴定[J].植物遗传资源学报,2013,14(2):311-316.
复制
文章指标
- 点击次数:2174
- 下载次数: 3148
- HTML阅读次数: 0
历史
- 收稿日期:2012-02-21
- 最后修改日期:2012-10-08
- 录用日期:2012-11-16
- 在线发布日期: 2013-02-01
文章二维码
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司